Frmd7 expression in developing mouse brain
Frmd7 expression in developing mouse brain
Aims: Mutations in the FERM domain containing 7 (FRMD7) genes are known to cause a significant number of cases of congenital idiopathic nystagmus (CIN). Only limited expression data exist suggesting low levels of expression in all tissues. In this study, we assess the expression profile of the murine homologue of FRMD7(Frmd7) in tissue from three murine organs during development.
Methods: cDNA was extracted from heart, lung, and brain tissues of MF-1 mice at 12 developmental time points, embryonic days 11–19, postnatal days 1 and 8, and from adult mice. Relative expression of Frmd7mRNA was calculated using quantitative real-time PCR techniques with two normalising genes (Gapdhand Actb).
Results: Expression of Frmd7was low in all tissues consistent with earlier reports. In heart and lung tissues, expression remained very low with an increase only in adult samples. In brain tissue, expression levels were higher at all time points with a significant increase at embryonic day 18, with no gender-specific influence on Frmd7expression.
Conclusions: Frmd7is expressed at low levels in all tissues studied suggesting a role in many tissue types. However, higher overall expression and a sharp increase at ED18 in the murine brain suggest a different role in this tissue.
Earlier studies have shown that genes expressed in the murine brain during development exhibit temporal functional clustering. The temporal pattern of Frmd7 expression found in this study mirrors that of genes involved in synapse formation/function, and genes related to axon growth/guidance. This suggests a role for Frmd7 in these processes and should direct further expression studies.
nystagmus, expression, frmd7, developing mouse brain
165-169
Self, J.
0f6efc58-ae24-4667-b8d6-6fafa849e389
Haitchi, H.M.
68dadb29-305d-4236-884f-e9c93f4d78fe
Griffiths, H.
a097fdaa-d3d6-49a9-9c69-0e6e5a5d518b
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Lotery, A.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
January 2010
Self, J.
0f6efc58-ae24-4667-b8d6-6fafa849e389
Haitchi, H.M.
68dadb29-305d-4236-884f-e9c93f4d78fe
Griffiths, H.
a097fdaa-d3d6-49a9-9c69-0e6e5a5d518b
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Lotery, A.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
Self, J., Haitchi, H.M., Griffiths, H., Holgate, S.T., Davies, D.E. and Lotery, A.
(2010)
Frmd7 expression in developing mouse brain.
Eye, 24 (1), .
(doi:10.1038/eye.2009.44).
(PMID:19265863)
Abstract
Aims: Mutations in the FERM domain containing 7 (FRMD7) genes are known to cause a significant number of cases of congenital idiopathic nystagmus (CIN). Only limited expression data exist suggesting low levels of expression in all tissues. In this study, we assess the expression profile of the murine homologue of FRMD7(Frmd7) in tissue from three murine organs during development.
Methods: cDNA was extracted from heart, lung, and brain tissues of MF-1 mice at 12 developmental time points, embryonic days 11–19, postnatal days 1 and 8, and from adult mice. Relative expression of Frmd7mRNA was calculated using quantitative real-time PCR techniques with two normalising genes (Gapdhand Actb).
Results: Expression of Frmd7was low in all tissues consistent with earlier reports. In heart and lung tissues, expression remained very low with an increase only in adult samples. In brain tissue, expression levels were higher at all time points with a significant increase at embryonic day 18, with no gender-specific influence on Frmd7expression.
Conclusions: Frmd7is expressed at low levels in all tissues studied suggesting a role in many tissue types. However, higher overall expression and a sharp increase at ED18 in the murine brain suggest a different role in this tissue.
Earlier studies have shown that genes expressed in the murine brain during development exhibit temporal functional clustering. The temporal pattern of Frmd7 expression found in this study mirrors that of genes involved in synapse formation/function, and genes related to axon growth/guidance. This suggests a role for Frmd7 in these processes and should direct further expression studies.
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Published date: January 2010
Keywords:
nystagmus, expression, frmd7, developing mouse brain
Identifiers
Local EPrints ID: 193065
URI: http://eprints.soton.ac.uk/id/eprint/193065
ISSN: 0950-222X
PURE UUID: 4522fe0e-07f1-4221-8a9e-73dc896614ba
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Date deposited: 12 Jul 2011 08:40
Last modified: 15 Mar 2024 03:24
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Author:
H. Griffiths
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