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A diagnostic EIA for detection of the prevalent SRSV strain in United Kingdom outbreaks of gastroenteritis.

Vipond, I. Barry, Pelosi, Emmanuela, Williams, Judith, Ashley, Charles R., Lambden, Paul R., Clarke, Ian N. and Caul, E. Owen (2000) A diagnostic EIA for detection of the prevalent SRSV strain in United Kingdom outbreaks of gastroenteritis. Journal of Medical Virology, 61, (1), pp. 132-137. (doi:10.1002/(SICI)1096-9071(200005)61:1<132::AID-JMV21>3.0.CO;2-8). (PMID:10745245).

Record type: Article

Abstract

Small round structured viruses (SRSVs) are the major cause of outbreaks of gastroenteritis in the UK. Diagnosis is problematic due to insensitive electron microscopy (EM) or technically demanding reverse transcription polymerase chain reaction (RT-PCR) techniques. We have studied outbreaks of non-bacterial gastroenteritis using an EIA based upon recombinant capsid protein from the currently prevalent circulating strain of SRSV (Lordsdale Genotype II) and compared its performance against EM and RT-PCR assays. Faecal specimens sent to the Bristol Public Health Laboratory for outbreak investigation from December 1996 to December 1997 were applied retrospectively to the SRSV EIA and results compared with the routine EM and RT-PCR that had been carried out prospectively. Overall, the three tests identified SRSVs in specimens from 70% of the outbreaks (213/305) investigated. Of the 213 total positive outbreaks, the EIA identified 71%, that compared favourably with EM (63%) and RT-PCR (84%). The Lordsdale Genotype II SRSV EIA provides a simple cost-effective assay that will for the first time make detection of currently circulating SRSV strains associated with UK outbreaks available to all routine laboratories. The EIA format makes the assay widely applicable to non-specialist laboratories, unlike the RT-PCR assay, and the improved sensitivity over EM will allow successful screening of UK outbreaks alongside commercial EIAs currently available for adenovirus, astrovirus and rotavirus. Furthermore, the assay will allow rapid identification of emerging SRSV strains.

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More information

Published date: 31 March 2000
Keywords: srsv, norwalk-like virus, lordsdale virus, enzyme immunoassay, recombinant capsid

Identifiers

Local EPrints ID: 194175
URI: http://eprints.soton.ac.uk/id/eprint/194175
ISSN: 0146-6615
PURE UUID: ef5cc69a-8b63-47c6-b362-5b63d6a2bf5d
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 25 Jul 2011 15:25
Last modified: 18 Jul 2017 11:27

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Contributors

Author: I. Barry Vipond
Author: Emmanuela Pelosi
Author: Judith Williams
Author: Charles R. Ashley
Author: Paul R. Lambden
Author: Ian N. Clarke ORCID iD
Author: E. Owen Caul

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