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Molecular characterization of human group C rotavirus genes 6, 7 and 9

James, Vivenna L.A., Lambden, Paul R., Deng, Yu, Caul, E. Owen and Clarke, Ian N. (1999) Molecular characterization of human group C rotavirus genes 6, 7 and 9 Journal of General Virology, 80, (12), pp. 3181-3187. (PMID:10567650).

Record type: Article


Genes 6, 7 and 9 of human group C rotavirus 'Bristol' strain, encoding non-structural proteins (NSP) 3, 1 and 2, respectively, were cloned and sequenced. Human group C rotavirus genome segment 6 is 1350 bp and contains a single ORF of 1231 nucleotides (encoding 402 amino acids). Genome segment 7 is 1270 bp and encodes a protein of 394 amino acids and genome segment 9 is 1037 bp and encodes a 312 amino acid protein. The human group C rotavirus genes 6, 7 and 9 showed 78, 67 and 88% sequence identity, respectively, to the corresponding porcine group C rotavirus genes. The derived protein sequences were compared with those of the porcine 'Cowden' group C and mammalian group A rotavirus strains. The human group C rotavirus NSP1 protein sequence is one amino acid longer than the porcine group C equivalent. In common with group A and porcine group C rotaviruses, the human group C rotavirus NSP1 protein has a zinc finger motif. Human group C rotavirus NSP2 has two hydrophobic heptad repeat regions, a basic, RNA-binding domain and a basic, proline-rich region. Human group C rotavirus NSP3 has both single- and double-stranded RNA-binding domains and several hydrophobic heptad repeat regions, one of which forms a leucine zipper. This work completes the molecular characterization of the non-structural proteins of a human group C rotavirus. Phylogenetic analysis of all the non-structural genes of group A, B and C rotaviruses suggests that these viruses have diverged at a constant rate from a common ancestor.

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Published date: December 1999


Local EPrints ID: 194179
ISSN: 0022-1317
PURE UUID: e43ce504-c87c-4a13-9d3d-070f222a96ec
ORCID for Ian N. Clarke: ORCID iD

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Date deposited: 25 Jul 2011 15:18
Last modified: 18 Jul 2017 11:27

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Author: Vivenna L.A. James
Author: Paul R. Lambden
Author: Yu Deng
Author: E. Owen Caul
Author: Ian N. Clarke ORCID iD

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