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Construction of an epitope vector utilising the diphtheria toxin B-subunit

Johnson, Nicholas, Pickett, Mark A., Watt, Peter J., Clarke, Ian N. and Heckels, John E. (1997) Construction of an epitope vector utilising the diphtheria toxin B-subunit FEMS Microbiology Letters, 146, (1), pp. 91-96. (doi:10.1016/S0378-1097(96)00459-4). (PMID:8997711).

Record type: Article


An immunogenic loop within the diphtheria toxin has been deleted from the B-subunit by a modification of the inverse polymerase chain reaction (IPCR) and replaced by a unique restriction endonuclease site. An oligonucleotide encoding an identified epitope sequence from the major outer membrane protein of Neisseria meningitidis of similar size and structure to that deleted has been introduced into the restriction site. Expression of the resulting chimeric B-subunit from Escherichia coli yielded a protein that was recognised by a panel of antibodies specific for the meningococcal epitope. Initial immunisation data suggest that this protein could elicit an antibody response against both diphtheria toxin and meningococcal proteins.

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Published date: 1 January 1997
Keywords: diphtheria toxin, vaccine, epitope, neisseria meningitidis


Local EPrints ID: 194273
ISSN: 0378-1097
PURE UUID: 9326d1f9-cb06-4cf8-8ed6-73908a254329
ORCID for Ian N. Clarke: ORCID iD

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Date deposited: 26 Jul 2011 14:29
Last modified: 18 Jul 2017 11:27

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Author: Nicholas Johnson
Author: Mark A. Pickett
Author: Peter J. Watt
Author: Ian N. Clarke ORCID iD
Author: John E. Heckels

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