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Construction of an epitope vector utilising the diphtheria toxin B-subunit

Construction of an epitope vector utilising the diphtheria toxin B-subunit
Construction of an epitope vector utilising the diphtheria toxin B-subunit
An immunogenic loop within the diphtheria toxin has been deleted from the B-subunit by a modification of the inverse polymerase chain reaction (IPCR) and replaced by a unique restriction endonuclease site. An oligonucleotide encoding an identified epitope sequence from the major outer membrane protein of Neisseria meningitidis of similar size and structure to that deleted has been introduced into the restriction site. Expression of the resulting chimeric B-subunit from Escherichia coli yielded a protein that was recognised by a panel of antibodies specific for the meningococcal epitope. Initial immunisation data suggest that this protein could elicit an antibody response against both diphtheria toxin and meningococcal proteins.
diphtheria toxin, vaccine, epitope, neisseria meningitidis
0378-1097
91-96
Johnson, Nicholas
b0c26909-6ebe-4979-aa83-2827ac095ad0
Pickett, Mark A.
8b9869fe-359f-4dd5-be8c-4edcb0d24eb3
Watt, Peter J.
8a27f505-e0f4-4663-a19a-ef7f156c8863
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31
Johnson, Nicholas
b0c26909-6ebe-4979-aa83-2827ac095ad0
Pickett, Mark A.
8b9869fe-359f-4dd5-be8c-4edcb0d24eb3
Watt, Peter J.
8a27f505-e0f4-4663-a19a-ef7f156c8863
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31

Johnson, Nicholas, Pickett, Mark A., Watt, Peter J., Clarke, Ian N. and Heckels, John E. (1997) Construction of an epitope vector utilising the diphtheria toxin B-subunit. FEMS Microbiology Letters, 146 (1), 91-96. (doi:10.1016/S0378-1097(96)00459-4). (PMID:8997711)

Record type: Article

Abstract

An immunogenic loop within the diphtheria toxin has been deleted from the B-subunit by a modification of the inverse polymerase chain reaction (IPCR) and replaced by a unique restriction endonuclease site. An oligonucleotide encoding an identified epitope sequence from the major outer membrane protein of Neisseria meningitidis of similar size and structure to that deleted has been introduced into the restriction site. Expression of the resulting chimeric B-subunit from Escherichia coli yielded a protein that was recognised by a panel of antibodies specific for the meningococcal epitope. Initial immunisation data suggest that this protein could elicit an antibody response against both diphtheria toxin and meningococcal proteins.

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More information

Published date: 1 January 1997
Keywords: diphtheria toxin, vaccine, epitope, neisseria meningitidis

Identifiers

Local EPrints ID: 194273
URI: https://eprints.soton.ac.uk/id/eprint/194273
ISSN: 0378-1097
PURE UUID: 9326d1f9-cb06-4cf8-8ed6-73908a254329
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 26 Jul 2011 14:29
Last modified: 20 Jul 2019 01:29

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Contributors

Author: Nicholas Johnson
Author: Mark A. Pickett
Author: Peter J. Watt
Author: Ian N. Clarke ORCID iD
Author: John E. Heckels

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