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Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches

Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches
Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches
We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT > GT > GG > AA approximate to TT approximate to TC > CA > GA > CC > GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n) > (GC)(n) > A(n)T(n) > (AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxy-ribose), but much weaker binding to G phi.
dna mismatches, dna recognition, mismatch repair, dna-base pairs, escherichia-coli, translocation mechanism, nucleotide-sequence, encoded protein, atp hydrolysis, repair system, hmuts-alpha, recognition, replication
1470-8728
627-633
Brown, James
212e608a-4b0a-4073-a06d-f60ed0310d3f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, James
212e608a-4b0a-4073-a06d-f60ed0310d3f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Brown, James, Brown, Tom and Fox, Keith R. (2001) Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches. Biochemical Journal, 354 (3), 627-633.

Record type: Article

Abstract

We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT > GT > GG > AA approximate to TT approximate to TC > CA > GA > CC > GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n) > (GC)(n) > A(n)T(n) > (AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxy-ribose), but much weaker binding to G phi.

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More information

Published date: 2001
Keywords: dna mismatches, dna recognition, mismatch repair, dna-base pairs, escherichia-coli, translocation mechanism, nucleotide-sequence, encoded protein, atp hydrolysis, repair system, hmuts-alpha, recognition, replication

Identifiers

Local EPrints ID: 19430
URI: https://eprints.soton.ac.uk/id/eprint/19430
ISSN: 1470-8728
PURE UUID: b619446f-4a84-4094-9694-def5657e6d48
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 14 Feb 2006
Last modified: 06 Jun 2018 13:16

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