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Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides

Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides
Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides
The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite.
fluorescence energy-transfer, dye-labeled primers, dna, hybridization, probes
0040-4039
2587-2591
Brown, LJ
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
May, JP
b54a262b-9f32-4435-8866-3b9c495294f3
Brown, T
a64aae36-bb30-42df-88a2-11be394e8c89
Brown, LJ
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
May, JP
b54a262b-9f32-4435-8866-3b9c495294f3
Brown, T
a64aae36-bb30-42df-88a2-11be394e8c89

Brown, LJ, May, JP and Brown, T (2001) Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides. Tetrahedron Letters, 42 (13), 2587-2591. (doi:10.1016/S0040-4039(01)00224-6).

Record type: Article

Abstract

The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite.

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More information

Published date: 26 March 2001
Keywords: fluorescence energy-transfer, dye-labeled primers, dna, hybridization, probes

Identifiers

Local EPrints ID: 19431
URI: http://eprints.soton.ac.uk/id/eprint/19431
ISSN: 0040-4039
PURE UUID: 33bea515-b930-4ae8-9b1b-b6e69fd2109e
ORCID for LJ Brown: ORCID iD orcid.org/0000-0002-5678-0814
ORCID for JP May: ORCID iD orcid.org/0000-0003-1651-130X

Catalogue record

Date deposited: 14 Feb 2006
Last modified: 09 Jan 2022 04:05

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Contributors

Author: LJ Brown ORCID iD
Author: JP May ORCID iD
Author: T Brown

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