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Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides

Brown, L.J., May, J.P. and Brown, T. (2001) Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides Tetrahedron Letters, 42, (13), pp. 2587-2591. (doi:10.1016/S0040-4039(01)00224-6).

Record type: Article

Abstract

The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite.

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More information

Published date: 26 March 2001
Keywords: fluorescence energy-transfer, dye-labeled primers, dna, hybridization, probes

Identifiers

Local EPrints ID: 19431
URI: http://eprints.soton.ac.uk/id/eprint/19431
ISSN: 0040-4039
PURE UUID: 33bea515-b930-4ae8-9b1b-b6e69fd2109e

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Date deposited: 14 Feb 2006
Last modified: 17 Jul 2017 16:31

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Contributors

Author: L.J. Brown
Author: J.P. May
Author: T. Brown

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