Developmental plasticity of human foetal femur-derived cells in pellet culture: self assembly of an osteoid shell around a cartilaginous core
Developmental plasticity of human foetal femur-derived cells in pellet culture: self assembly of an osteoid shell around a cartilaginous core
This study has examined the osteogenic and chondrogenic differentiation of human foetal femur-derived cells in 3-dimensional pellet cultures. After culture for 21-28 days in osteogenic media, the pellets acquired a unique configuration that consisted of an outer fibrous layer, an osteoid-like shell surrounding a cellular and cartilaginous region. This configuration is typical to the cross section of the foetal femurs at the same age and was not observed in pellets derived from adult human bone marrow stromal cells. Time course study showed that after 7-14 days, the cells of the inner cellular region were viable, proliferated rapidly, and were immuno-positive for c-myc, as well as for bone sialoprotein and type I collagen. After 21-28 days, the cells accumulated at the inner edge of the osteoid shell. The direction of osteoid formation thus differed from that of periosteal bone formation. Following micro-dissection of the human foetal femurs into epiphyses, bone cylinder and hypertrophic cartilage, epiphyseal chondrocytes and osteoblasts both gave rise to osteoid-shell forming cells. These studies demonstrate the developmental plasticity of human foetal skeletal and epiphyseal chondrocytes and suggest that the microenvironment modulates lineage commitment and matrix formation. Furthermore, this ex vivo model offers a new approach to delineate human bone development as well as a model with potential application for evaluation of therapeutic compounds for bone formation.
human foetal cells, stem cells, differentiation, bone development, pellet culture, bone model, tissue engineering, self assembly, 3d model
558-567
El-Serafi, A.T.
ecf93d5a-8b3f-4b03-8718-5a3996078885
Wilson, D.I.
1500fca1-7082-4271-95f4-691f1d1252a2
Roach, H.I.
1c0cf1f8-15dc-49a8-aad8-9ed3fd13c05b
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
20 June 2011
El-Serafi, A.T.
ecf93d5a-8b3f-4b03-8718-5a3996078885
Wilson, D.I.
1500fca1-7082-4271-95f4-691f1d1252a2
Roach, H.I.
1c0cf1f8-15dc-49a8-aad8-9ed3fd13c05b
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
El-Serafi, A.T., Wilson, D.I., Roach, H.I. and Oreffo, R.O.C.
(2011)
Developmental plasticity of human foetal femur-derived cells in pellet culture: self assembly of an osteoid shell around a cartilaginous core.
European Cells & Materials, 21, .
(PMID:21710446)
Abstract
This study has examined the osteogenic and chondrogenic differentiation of human foetal femur-derived cells in 3-dimensional pellet cultures. After culture for 21-28 days in osteogenic media, the pellets acquired a unique configuration that consisted of an outer fibrous layer, an osteoid-like shell surrounding a cellular and cartilaginous region. This configuration is typical to the cross section of the foetal femurs at the same age and was not observed in pellets derived from adult human bone marrow stromal cells. Time course study showed that after 7-14 days, the cells of the inner cellular region were viable, proliferated rapidly, and were immuno-positive for c-myc, as well as for bone sialoprotein and type I collagen. After 21-28 days, the cells accumulated at the inner edge of the osteoid shell. The direction of osteoid formation thus differed from that of periosteal bone formation. Following micro-dissection of the human foetal femurs into epiphyses, bone cylinder and hypertrophic cartilage, epiphyseal chondrocytes and osteoblasts both gave rise to osteoid-shell forming cells. These studies demonstrate the developmental plasticity of human foetal skeletal and epiphyseal chondrocytes and suggest that the microenvironment modulates lineage commitment and matrix formation. Furthermore, this ex vivo model offers a new approach to delineate human bone development as well as a model with potential application for evaluation of therapeutic compounds for bone formation.
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Published date: 20 June 2011
Keywords:
human foetal cells, stem cells, differentiation, bone development, pellet culture, bone model, tissue engineering, self assembly, 3d model
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Local EPrints ID: 194375
URI: http://eprints.soton.ac.uk/id/eprint/194375
PURE UUID: 0ad4e50a-fb15-4968-9f97-f1244c62fbfb
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Date deposited: 27 Jul 2011 13:07
Last modified: 23 Jul 2022 01:46
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Author:
A.T. El-Serafi
Author:
H.I. Roach
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