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Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples

Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples
Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples
We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, el simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.
polymerase-chain-reaction, nested case-control, carcinoma in-situ, cervical-cancer, viral load, high-risk, dna, worldwide, variants
0095-1137
3204-3212
Hart, Keith W.
4df11d74-411a-45f7-b5b7-251f6a6a12d8
Williams, O. Martin
8b746e99-67dc-4e66-bdb0-492342f4fcfe
Thelwell, Nicola
f4f0808c-02a5-4380-a060-7537ad3b27ec
Fiander, Alison N.
438cc01f-aaa5-4772-85ef-10f19b0f2fe4
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Borysiewicz, Lesek K.
b205c81f-615c-4e1e-b748-acf9d0d9e2e4
Gelder, Colin M.
0916956b-21fe-444e-8805-71748ede969e
Hart, Keith W.
4df11d74-411a-45f7-b5b7-251f6a6a12d8
Williams, O. Martin
8b746e99-67dc-4e66-bdb0-492342f4fcfe
Thelwell, Nicola
f4f0808c-02a5-4380-a060-7537ad3b27ec
Fiander, Alison N.
438cc01f-aaa5-4772-85ef-10f19b0f2fe4
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Borysiewicz, Lesek K.
b205c81f-615c-4e1e-b748-acf9d0d9e2e4
Gelder, Colin M.
0916956b-21fe-444e-8805-71748ede969e

Hart, Keith W., Williams, O. Martin, Thelwell, Nicola, Fiander, Alison N., Brown, Tom, Borysiewicz, Lesek K. and Gelder, Colin M. (2001) Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. Journal of Clinical Microbiology, 39 (9), 3204-3212. (doi:10.1128/JCM.39.9.3204-3212.2001).

Record type: Article

Abstract

We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, el simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.

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More information

Published date: 1 September 2001
Keywords: polymerase-chain-reaction, nested case-control, carcinoma in-situ, cervical-cancer, viral load, high-risk, dna, worldwide, variants

Identifiers

Local EPrints ID: 19512
URI: http://eprints.soton.ac.uk/id/eprint/19512
ISSN: 0095-1137
PURE UUID: a4268cd5-24b0-416c-b907-8f50fe6eff56

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Date deposited: 15 Feb 2006
Last modified: 15 Jul 2019 19:27

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Contributors

Author: Keith W. Hart
Author: O. Martin Williams
Author: Nicola Thelwell
Author: Alison N. Fiander
Author: Tom Brown
Author: Lesek K. Borysiewicz
Author: Colin M. Gelder

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