The University of Southampton
University of Southampton Institutional Repository

On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification
On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification
Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.
2127-2133
Tsaloglou, Maria-Nefeli
99ab30ba-15da-4d25-86ba-608d127f8369
Bahi, Mahadji M.
424b12dc-a6d1-446c-b0aa-66c6aa22c449
Waugh, Edward M.
bf1debbf-0db9-43d3-84a5-044a22c52748
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew
6f633ca2-298f-48ee-a025-ce52dd62124f
Tsaloglou, Maria-Nefeli
99ab30ba-15da-4d25-86ba-608d127f8369
Bahi, Mahadji M.
424b12dc-a6d1-446c-b0aa-66c6aa22c449
Waugh, Edward M.
bf1debbf-0db9-43d3-84a5-044a22c52748
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Mowlem, Matthew
6f633ca2-298f-48ee-a025-ce52dd62124f

Tsaloglou, Maria-Nefeli, Bahi, Mahadji M., Waugh, Edward M., Morgan, Hywel and Mowlem, Matthew (2011) On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification. Analytical Methods, 3 (9), 2127-2133. (doi:10.1039/C1AY05164D).

Record type: Article

Abstract

Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.

Full text not available from this repository.

More information

Published date: 17 August 2011
Organisations: Electronics & Computer Science, Ocean Technology and Engineering

Identifiers

Local EPrints ID: 195275
URI: https://eprints.soton.ac.uk/id/eprint/195275
PURE UUID: 4c186274-9684-4a0d-a3f1-1f9c4f3303f8
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 18 Aug 2011 08:56
Last modified: 20 Jul 2019 00:59

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×