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On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification

Record type: Article

Development of integrated systems for nucleic acid analyses is mainly driven by the requirement for fast and simple clinical and environmental diagnostics. The need for affordable and effective point-of-care diagnosis has inspired an entire field of biotechnology in micro and nano-fluidics. We are developing a microfluidic system that has individual sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time RNA detection. The system is being developed to analyse the rbcL gene of phytoplankton Karenia brevis, a species responsible for harmful algal blooms. This integrated system will perform sub-cellular analysis of RNA using nucleic acid sequence-based amplification and would be used in large scale biochemical analysis and experimentation. The device could potentially be used for the detection of any species with a known target nucleic acid sequence for in situ environmental monitoring, forensics or clinical diagnostics.

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Citation

Tsaloglou, Maria-Nefeli, Bahi, Mahadji M., Waugh, Edward M., Morgan, Hywel and Mowlem, Matthew (2011) On-chip real-time nucleic acid sequence-based amplification for RNA detection and amplification Analytical Methods, 3, (9), pp. 2127-2133. (doi:10.1039/C1AY05164D).

More information

Published date: 17 August 2011
Organisations: Electronics & Computer Science, Ocean Technology and Engineering

Identifiers

Local EPrints ID: 195275
URI: http://eprints.soton.ac.uk/id/eprint/195275
PURE UUID: 4c186274-9684-4a0d-a3f1-1f9c4f3303f8
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 18 Aug 2011 08:56
Last modified: 18 Jul 2017 11:24

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Contributors

Author: Maria-Nefeli Tsaloglou
Author: Mahadji M. Bahi
Author: Edward M. Waugh
Author: Hywel Morgan ORCID iD
Author: Matthew Mowlem

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