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Developmental changes in rat surfactant lipidomics in the context of species variability.

Developmental changes in rat surfactant lipidomics in the context of species variability.
Developmental changes in rat surfactant lipidomics in the context of species variability.
Lung surfactant comprises mainly phosphatidylcholine (PC) species together with phosphatidylglycerols and surfactant proteins (SP) SP-A to -D. Changes in the concentrations of its principal components dipalmitoyl-PC, palmitoylmyristoyl-PC, palmitoylpalmitoleoyl-PC relative to developmental, structural and physiological differences are only partially understood. Particularly, their attribution to differences in air-liquid interface curvature, compared with dynamic parameters, such as respiratory rate, are controversial.

We postulated that during alveolarization the changes in these principal PC components of surfactant differ from those in other phospholipid parameters, and that across endothermic vertebrates their concentrations are related to lung physiology rather than structure. We therefore investigated in rats from postnatal day (d)1 to d42 the pattern of surfactant phospholipids relative to alveolarization (d4-d14), and we discuss these changes in terms of molecular adaptation to pulmonary structure or physiology. Contrary to mammals with advanced alveolarization and increased respiratory rate (RR) at term, concentrations of dipalmitoyl-PC (49-52%) and palmitoylmyristoyl-PC (7-9%) in lung lavage fluid were identical at d1 and d42. At d7-d14, when in rats RR is increased, palmitoyl-myristoyl-PC transiently increased by 2.5- to 3.9-fold at the expense of dipalmitoyl-PC (-32% to 34%) and palmitoyl-palmitoleoyl-PC (-16%). Other lipidomic changes followed essentially different patterns of increase or decrease. Palmitoyl-myristoyl-PC was increased in large aggregates suggesting that it is an integral component of active surfactant.

In the overall context of vertebrates, irrespective of age and lung structure, fractions of palmitoyl-myristoyl-PC, dipalmitoyl-PC and palmitoyl-palmitoleoyl-PC correlate with differences in RR rather than alveolar curvature. In adult mammals, however, only concentrations of palmitoyl-palmitoleoyl-PC correlate with RR.
lung development, surfactant, rat lung, respiration, comparative biology, endotherm, mammals, birds, lipidomics
8755-6863
794-804
Bernhard, Wolfgang
a93ed9d7-1f32-42e8-bf9c-c9f0f14ad2a2
Schmiedl, Andreas
07208515-3487-4a1e-8991-314a2ccd99d0
Koster, Grielof
e404c38a-6f48-430a-adf0-5208228cb9e7
Orgeig, Sandra
5f9ef159-9b3e-4f84-8b10-a24398529957
Acevedo, Christa
f21b973f-930f-4c7f-92d9-911371c65342
Poets, Christian F.
611dc9d4-8d1b-4f68-8d82-c18e13fb92ba
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Bernhard, Wolfgang
a93ed9d7-1f32-42e8-bf9c-c9f0f14ad2a2
Schmiedl, Andreas
07208515-3487-4a1e-8991-314a2ccd99d0
Koster, Grielof
e404c38a-6f48-430a-adf0-5208228cb9e7
Orgeig, Sandra
5f9ef159-9b3e-4f84-8b10-a24398529957
Acevedo, Christa
f21b973f-930f-4c7f-92d9-911371c65342
Poets, Christian F.
611dc9d4-8d1b-4f68-8d82-c18e13fb92ba
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66

Bernhard, Wolfgang, Schmiedl, Andreas, Koster, Grielof, Orgeig, Sandra, Acevedo, Christa, Poets, Christian F. and Postle, Anthony D. (2007) Developmental changes in rat surfactant lipidomics in the context of species variability. Pediatric Pulmonology, 42 (9), 794-804. (doi:10.1002/ppul.20657). (PMID:17659602)

Record type: Article

Abstract

Lung surfactant comprises mainly phosphatidylcholine (PC) species together with phosphatidylglycerols and surfactant proteins (SP) SP-A to -D. Changes in the concentrations of its principal components dipalmitoyl-PC, palmitoylmyristoyl-PC, palmitoylpalmitoleoyl-PC relative to developmental, structural and physiological differences are only partially understood. Particularly, their attribution to differences in air-liquid interface curvature, compared with dynamic parameters, such as respiratory rate, are controversial.

We postulated that during alveolarization the changes in these principal PC components of surfactant differ from those in other phospholipid parameters, and that across endothermic vertebrates their concentrations are related to lung physiology rather than structure. We therefore investigated in rats from postnatal day (d)1 to d42 the pattern of surfactant phospholipids relative to alveolarization (d4-d14), and we discuss these changes in terms of molecular adaptation to pulmonary structure or physiology. Contrary to mammals with advanced alveolarization and increased respiratory rate (RR) at term, concentrations of dipalmitoyl-PC (49-52%) and palmitoylmyristoyl-PC (7-9%) in lung lavage fluid were identical at d1 and d42. At d7-d14, when in rats RR is increased, palmitoyl-myristoyl-PC transiently increased by 2.5- to 3.9-fold at the expense of dipalmitoyl-PC (-32% to 34%) and palmitoyl-palmitoleoyl-PC (-16%). Other lipidomic changes followed essentially different patterns of increase or decrease. Palmitoyl-myristoyl-PC was increased in large aggregates suggesting that it is an integral component of active surfactant.

In the overall context of vertebrates, irrespective of age and lung structure, fractions of palmitoyl-myristoyl-PC, dipalmitoyl-PC and palmitoyl-palmitoleoyl-PC correlate with differences in RR rather than alveolar curvature. In adult mammals, however, only concentrations of palmitoyl-palmitoleoyl-PC correlate with RR.

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More information

Published date: September 2007
Keywords: lung development, surfactant, rat lung, respiration, comparative biology, endotherm, mammals, birds, lipidomics
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 195583
URI: https://eprints.soton.ac.uk/id/eprint/195583
ISSN: 8755-6863
PURE UUID: 6cfcc91c-bff6-4204-ad60-560c432c9123
ORCID for Anthony D. Postle: ORCID iD orcid.org/0000-0001-7361-0756

Catalogue record

Date deposited: 23 Aug 2011 13:24
Last modified: 06 Jun 2018 13:20

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