The University of Southampton
University of Southampton Institutional Repository

Repeated high-dose inhalation allergen challenge in asthma

Grainge, Christophe and Howarth, Peter (2011) Repeated high-dose inhalation allergen challenge in asthma Clinical Respiratory Journal, 5, (3), pp. 150-155. (doi:10.1111/j.1752-699X.2010.00212.x). (PMID:21679350).

Record type: Article


Introduction:? Inhalation allergen challenge in humans is used to investigate lung pathophysiology and responses to novel therapies. However, the single high-dose allergen challenges that are commonly performed do not mimic repeated symptomatic environmental allergen exposure.

Objectives:? To develop and evaluate the safety of a repeated high-dose symptomatic inhalation allergen challenge model.

Methods:? Sixteen subjects with atopic asthma were recruited. Each underwent three inhalation allergen challenges using house dust mite (Dermatophagoides pteronyssinus) antigen at 48-h intervals with a target of symptom induction and an early asthmatic reaction fall in forced expiratory volume in 1 s (FEV1) of 15% from baseline.

Results:? All of the subjects completed the three-challenge protocol and the target immediate airway bronchoconstrictor response was achieved in all the subjects at all challenges. There were no adverse events recorded. The early asthmatic reaction was similar for the three challenges whether measured as mean maximal fall in FEV1 or mean area under the curve. The late asthmatic reaction was also similar over the three challenges with no evidence of priming or desensitisation. Symptom scores and reliever medication use significantly increased over the time of the challenges. Baseline lung function and reversibility was unchanged 4 days after the last challenge.

Conclusion:? We demonstrate that repeated high-dose inhaled house dust mite allergen challenge in human volunteers with mild asthma is safe, repeatable and acceptable. This allows the use of this model in further studies focused on understanding the pathophysiology of allergen induced asthma and the impact of therapeutic interventions.

Full text not available from this repository.

More information

Published date: 7 June 2011
Keywords: adult, allergens, antigens, dermatophagoides, asthma, bronchial provocation tests, humans
Organisations: Clinical & Experimental Sciences


Local EPrints ID: 195635
ISSN: 1752-6981
PURE UUID: 8d339a6f-15b7-4d48-a687-5ef26065aac1

Catalogue record

Date deposited: 24 Aug 2011 12:35
Last modified: 18 Jul 2017 11:24

Export record



Author: Christophe Grainge
Author: Peter Howarth

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.