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Solid-phase synthesis of peptide-dendrimer conjugates for an investigation of integrin binding

Solid-phase synthesis of peptide-dendrimer conjugates for an investigation of integrin binding
Solid-phase synthesis of peptide-dendrimer conjugates for an investigation of integrin binding
Peptide-dendrimer conjugates, bearing up to eight peptide ligands (LDV) targeted against the alpha (4)beta integrin VLA-4 were prepared by solid-phase synthesis. Solid phase synthesis allowing the dendrimer conjugates to be prepared in high purity for an investigation of the multivalent effect in ligand integrin interactions. An ELISA was used to screen the peptide-dendrimer conjugates for their ability to compete with a biotinylated peptide component of the CS1 region of fibronectin. The inhibition potential of the different generation peptide-dendrimer conjugates was found to increase with higher generations.
cell-adhesion, recognition, fibronectin, generation, sialosides, inhibitors, libraries, sequence, rgd
1424-6376
46-53
Monaghan, Seán
fad6dcfe-6c4c-4f28-9cd6-1b7da45f0758
Griffith-Johnson, David
976a3360-c6ca-4d3b-8122-bde015b5cbf0
Matthews, Ian
3cc6e23b-692f-4153-b67a-db41dde454cb
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Monaghan, Seán
fad6dcfe-6c4c-4f28-9cd6-1b7da45f0758
Griffith-Johnson, David
976a3360-c6ca-4d3b-8122-bde015b5cbf0
Matthews, Ian
3cc6e23b-692f-4153-b67a-db41dde454cb
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900

Monaghan, Seán, Griffith-Johnson, David, Matthews, Ian and Bradley, Mark (2001) Solid-phase synthesis of peptide-dendrimer conjugates for an investigation of integrin binding. Arkivoc, 2001 (Part (x)), 46-53.

Record type: Article

Abstract

Peptide-dendrimer conjugates, bearing up to eight peptide ligands (LDV) targeted against the alpha (4)beta integrin VLA-4 were prepared by solid-phase synthesis. Solid phase synthesis allowing the dendrimer conjugates to be prepared in high purity for an investigation of the multivalent effect in ligand integrin interactions. An ELISA was used to screen the peptide-dendrimer conjugates for their ability to compete with a biotinylated peptide component of the CS1 region of fibronectin. The inhibition potential of the different generation peptide-dendrimer conjugates was found to increase with higher generations.

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More information

Published date: 2001
Keywords: cell-adhesion, recognition, fibronectin, generation, sialosides, inhibitors, libraries, sequence, rgd

Identifiers

Local EPrints ID: 19576
URI: http://eprints.soton.ac.uk/id/eprint/19576
ISSN: 1424-6376
PURE UUID: 501d9c48-dcb0-4489-ab6f-6dd6a81a947b

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Date deposited: 14 Feb 2006
Last modified: 09 Jan 2022 04:55

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Contributors

Author: Seán Monaghan
Author: David Griffith-Johnson
Author: Ian Matthews
Author: Mark Bradley

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