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Type II cytochrome c(3) from desulfovibrio africanus: a structure-function correlation

Type II cytochrome c(3) from desulfovibrio africanus: a structure-function correlation
Type II cytochrome c(3) from desulfovibrio africanus: a structure-function correlation
A type II tetrahaem cytochrome c3 (TplI-c3) was isolated from the periplasmic space of Desulfovibrio africanus, a sulfate-reducing bacter'm m~. Although structurally si.milar to the .well charact.ensed type I. cytochrome s c3 (TpI-c3), this protein displays distinct properties z-5. In particular, it is part of a polycistronic operon coding for transmembrane redox proteins 4, suggesting a different physiological function from that performed by TpI-c3. The thermodynamic parameters of the haems and of an acid-base centre in the TplI-c3 were determined by using the NMR and visible spectroscopies and by fitting the data to a five centres thermodynamic model 6. The thermodynamic features are correlated with the redox-linked structural differences reported in the literature3: positive cooperativity between the haems is not observed. The redox-Bohr effect is strongly associated with haem II instead of haem I as in the case for all of the TpI-c3s 5' 6. These novel functional properties are in agreement with the structural differences between TpI- and TplI-c3 and the redox-linked structural changes observed and may be relevant for the difference in the physiological function proposed for TpII-c3s vs TpI-c3s TM
0162-0134
376
Pereira, Patricia M.
b7e35a13-ed80-4e5e-8b94-174941364c70
Catarino, Teresa
608a4400-1611-4798-bf27-7472e7c6a476
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Xavier, Antonio V.
28457543-8cb4-48d4-b66a-d6524d2b3f13
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pereira, Patricia M.
b7e35a13-ed80-4e5e-8b94-174941364c70
Catarino, Teresa
608a4400-1611-4798-bf27-7472e7c6a476
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Xavier, Antonio V.
28457543-8cb4-48d4-b66a-d6524d2b3f13
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6

Pereira, Patricia M., Catarino, Teresa, Turner, David L., Xavier, Antonio V. and Louro, Ricardo O. (2001) Type II cytochrome c(3) from desulfovibrio africanus: a structure-function correlation. Journal of Inorganic Biochemistry, 86 (1), 376. (doi:10.1016/S0162-0134(01)00284-7).

Record type: Article

Abstract

A type II tetrahaem cytochrome c3 (TplI-c3) was isolated from the periplasmic space of Desulfovibrio africanus, a sulfate-reducing bacter'm m~. Although structurally si.milar to the .well charact.ensed type I. cytochrome s c3 (TpI-c3), this protein displays distinct properties z-5. In particular, it is part of a polycistronic operon coding for transmembrane redox proteins 4, suggesting a different physiological function from that performed by TpI-c3. The thermodynamic parameters of the haems and of an acid-base centre in the TplI-c3 were determined by using the NMR and visible spectroscopies and by fitting the data to a five centres thermodynamic model 6. The thermodynamic features are correlated with the redox-linked structural differences reported in the literature3: positive cooperativity between the haems is not observed. The redox-Bohr effect is strongly associated with haem II instead of haem I as in the case for all of the TpI-c3s 5' 6. These novel functional properties are in agreement with the structural differences between TpI- and TplI-c3 and the redox-linked structural changes observed and may be relevant for the difference in the physiological function proposed for TpII-c3s vs TpI-c3s TM

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Published date: 1 August 2001

Identifiers

Local EPrints ID: 19590
URI: http://eprints.soton.ac.uk/id/eprint/19590
ISSN: 0162-0134
PURE UUID: 6a7c62fd-af8f-417d-b9a4-1e7e8f758693

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Date deposited: 14 Feb 2006
Last modified: 17 Jul 2017 16:31

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