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Redox and redox-Bohr cooperativity studies in a new tetrahaem cytochrome isolated from Shewanella frigidimarina

Redox and redox-Bohr cooperativity studies in a new tetrahaem cytochrome isolated from Shewanella frigidimarina
Redox and redox-Bohr cooperativity studies in a new tetrahaem cytochrome isolated from Shewanella frigidimarina
During anaerobic growth the facultatively anaerobic bacterium, Shewanella frigidimarina NCIMB400, Sf produces a small tetrahaem cytochrome (86 residues) that is involved in electron transfer to Fe(III), a terminal respiratory oxidant of this microorganism ~. A 3D structure model of the reduced form of this cytochrome was determined by NMR ~. The haem core architecture of Sftetrahaem cytochrome differs from that found in all small tetrahaem cytochromes c3 so far isolated from strict anaerobes, but has some similarity with to the N-terminal cytochrome domain of the flavocytochrome c~ isolated from Sf 3 Based on the 3D structure model and in NMR redox titrations it was possible to assign the order of oxidation of the haems 2. NMR and visible redox titrations were also performed at different pH values to determine both the haem potentials, as well as the redox and redox-Bohr cooperativities in the Sftetrahaem cytochrome.
0162-0134
378
Pessanha, Miguel
7e563109-5e0f-4949-b0cc-9cab7a8e2809
Brennan, Lorraine
efa9251d-9605-48ae-b1b0-27129a4a9270
Xavier, Antonio V.
28457543-8cb4-48d4-b66a-d6524d2b3f13
Cuthbertson, Pauline M.
622e17ad-e1c0-42dc-b611-a3f5b0bc2180
Reid, Graem A.
37d35b11-40ce-48c5-a68e-f6ce04cd4037
Chapman, Stephen K.
c8004be1-78bc-406c-8150-2cc282f910fa
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Salgueiro, Carlos A.
a9805ac9-e610-42ca-bb85-ef4e288eeffb
Pessanha, Miguel
7e563109-5e0f-4949-b0cc-9cab7a8e2809
Brennan, Lorraine
efa9251d-9605-48ae-b1b0-27129a4a9270
Xavier, Antonio V.
28457543-8cb4-48d4-b66a-d6524d2b3f13
Cuthbertson, Pauline M.
622e17ad-e1c0-42dc-b611-a3f5b0bc2180
Reid, Graem A.
37d35b11-40ce-48c5-a68e-f6ce04cd4037
Chapman, Stephen K.
c8004be1-78bc-406c-8150-2cc282f910fa
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Salgueiro, Carlos A.
a9805ac9-e610-42ca-bb85-ef4e288eeffb

Pessanha, Miguel, Brennan, Lorraine, Xavier, Antonio V., Cuthbertson, Pauline M., Reid, Graem A., Chapman, Stephen K., Turner, David L. and Salgueiro, Carlos A. (2001) Redox and redox-Bohr cooperativity studies in a new tetrahaem cytochrome isolated from Shewanella frigidimarina. Journal of Inorganic Biochemistry, 86 (1), 378.

Record type: Article

Abstract

During anaerobic growth the facultatively anaerobic bacterium, Shewanella frigidimarina NCIMB400, Sf produces a small tetrahaem cytochrome (86 residues) that is involved in electron transfer to Fe(III), a terminal respiratory oxidant of this microorganism ~. A 3D structure model of the reduced form of this cytochrome was determined by NMR ~. The haem core architecture of Sftetrahaem cytochrome differs from that found in all small tetrahaem cytochromes c3 so far isolated from strict anaerobes, but has some similarity with to the N-terminal cytochrome domain of the flavocytochrome c~ isolated from Sf 3 Based on the 3D structure model and in NMR redox titrations it was possible to assign the order of oxidation of the haems 2. NMR and visible redox titrations were also performed at different pH values to determine both the haem potentials, as well as the redox and redox-Bohr cooperativities in the Sftetrahaem cytochrome.

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Published date: 1 August 2001

Identifiers

Local EPrints ID: 19593
URI: http://eprints.soton.ac.uk/id/eprint/19593
ISSN: 0162-0134
PURE UUID: 4ef1b8d4-8895-4507-9c26-ba7ee7ed6d12
ORCID for Graem A. Reid: ORCID iD orcid.org/0000-0001-5349-3468

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Date deposited: 14 Feb 2006
Last modified: 14 Jul 2018 00:37

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Contributors

Author: Miguel Pessanha
Author: Lorraine Brennan
Author: Antonio V. Xavier
Author: Pauline M. Cuthbertson
Author: Graem A. Reid ORCID iD
Author: Stephen K. Chapman
Author: David L. Turner
Author: Carlos A. Salgueiro

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