Zhang, Y., Zhang, W., Johnston, A., Newman, T.A., Pyykko, I. and Zou, J.
Comparison of the distribution pattern of PEG-b-PCL polymersomes delivered into the rat inner ear via different methods
Acta Oto-Laryngologica (PMID:21905960).
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Cochleostomy is the most efficient approach in delivering PEG-b-PCL polymersomes (PMs) to the inner ear. PMs 10 can be delivered to the vestibule by transtympanic injection or cochleostomy. Objective: To evaluate the efficiency of delivering 11 PEG-b-PCL PMs into the inner ear using different approaches. Methods: The PEG-b-PCL PMs were administered either by 12 sustained topical round window membrane (RWM) delivery using gelatin sponge pledgets in combination with an osmotic 13 pump, transtympanic injection, or cochleostomy. The distribution of the PMs in the inner ear was observed by confocal 14 microscopy using either whole mount specimens or cryosections. Results: Cochleostomy resulted in distribution of the PMs in 15 the spiral ligament (SL), mesothelial cells beneath the organ of Corti, supporting cells in the organ of Corti, and spiral ganglion 16 cells (SGCs). Transtympanic injection induced uptake of the PMs in the SL and mesothelial cells beneath the organ of Corti. 17 Topical administration showed distribution of the PMs only in the SL. In the vestibulum, transtympanic injection and 18 cochleostomy induced more distribution of the PMs than did topical RWM delivery (p < 0.05, Kruskal-Wallis test).
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