The University of Southampton
University of Southampton Institutional Repository

Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency

Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency
Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency
There is currently an unmet need for the supply of autologous, patient-specific stem cells for regenerative therapies in the clinic. Mesenchymal stem cell differentiation can be driven by the material/cell interface suggesting a unique strategy to manipulate stem cells in the absence of complex soluble chemistries or cellular reprogramming. However, so far the derivation and identification of surfaces that allow retention of multipotency of this key regenerative cell type have remained elusive. Adult stem cells spontaneously differentiate in culture, resulting in a rapid diminution of the multipotent cell population and their regenerative capacity. Here we identify a nanostructured surface that retains stem-cell phenotype and maintains stem-cell growth over eight weeks. Furthermore, the study implicates a role for small RNAs in repressing key cell signalling and metabolomic pathways, demonstrating the potential of surfaces as non-invasive tools with which to address the stem cell niche.

1476-1122
637-644
McMurray, Rebecca J.
2e4cf997-4e4a-4daf-9d49-09b83abde8cf
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Tsimbouri, P. Monica
e4ce3dfc-d952-4737-af2c-b1b06f9503df
Burgess, Karl V.
92de78f3-7b0d-4a93-9672-25bdc66cbb2b
McNamara, Laura E.
c5efd583-f6a3-4907-b357-1ccf4dba31cb
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Murawski, Kate
6d488c09-d594-4791-be13-9844f8ded26a
Kingham, Emmajayne
4ad3641f-bdca-464a-b5e8-cb1fc995409a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
McMurray, Rebecca J.
2e4cf997-4e4a-4daf-9d49-09b83abde8cf
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Tsimbouri, P. Monica
e4ce3dfc-d952-4737-af2c-b1b06f9503df
Burgess, Karl V.
92de78f3-7b0d-4a93-9672-25bdc66cbb2b
McNamara, Laura E.
c5efd583-f6a3-4907-b357-1ccf4dba31cb
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Murawski, Kate
6d488c09-d594-4791-be13-9844f8ded26a
Kingham, Emmajayne
4ad3641f-bdca-464a-b5e8-cb1fc995409a
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc

McMurray, Rebecca J., Gadegaard, Nikolaj, Tsimbouri, P. Monica, Burgess, Karl V., McNamara, Laura E., Tare, Rahul, Murawski, Kate, Kingham, Emmajayne, Oreffo, Richard O.C. and Dalby, Matthew J. (2011) Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nature Materials, 10 (8), 637-644. (doi:10.1038/nmat3058). (PMID:21765399)

Record type: Article

Abstract

There is currently an unmet need for the supply of autologous, patient-specific stem cells for regenerative therapies in the clinic. Mesenchymal stem cell differentiation can be driven by the material/cell interface suggesting a unique strategy to manipulate stem cells in the absence of complex soluble chemistries or cellular reprogramming. However, so far the derivation and identification of surfaces that allow retention of multipotency of this key regenerative cell type have remained elusive. Adult stem cells spontaneously differentiate in culture, resulting in a rapid diminution of the multipotent cell population and their regenerative capacity. Here we identify a nanostructured surface that retains stem-cell phenotype and maintains stem-cell growth over eight weeks. Furthermore, the study implicates a role for small RNAs in repressing key cell signalling and metabolomic pathways, demonstrating the potential of surfaces as non-invasive tools with which to address the stem cell niche.

Text
Nature_Materials_2011.pdf - Version of Record
Restricted to Repository staff only
Request a copy

More information

Published date: 17 July 2011
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 197139
URI: http://eprints.soton.ac.uk/id/eprint/197139
ISSN: 1476-1122
PURE UUID: f36f3b61-d5aa-4b83-9215-f541360de12a
ORCID for Rahul Tare: ORCID iD orcid.org/0000-0001-8274-8837
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 19 Sep 2011 09:46
Last modified: 15 Mar 2024 03:20

Export record

Altmetrics

Contributors

Author: Rebecca J. McMurray
Author: Nikolaj Gadegaard
Author: P. Monica Tsimbouri
Author: Karl V. Burgess
Author: Laura E. McNamara
Author: Rahul Tare ORCID iD
Author: Kate Murawski
Author: Emmajayne Kingham
Author: Matthew J. Dalby

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×