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Demonstration of bacillus cereus in orthopaedic-implant-related infection with use of a multi-primer polymerase chain reaction-mass spectrometric assay: report of two cases

Demonstration of bacillus cereus in orthopaedic-implant-related infection with use of a multi-primer polymerase chain reaction-mass spectrometric assay: report of two cases
Demonstration of bacillus cereus in orthopaedic-implant-related infection with use of a multi-primer polymerase chain reaction-mass spectrometric assay: report of two cases
The recent advent of a novel coupled PCR-mass spectrometric technology, the Ibis T5000(Abbott Laboratories, Chicago, Illinois) offers multiple potential advantages for molecular detection of periprosthetic joint infection or other implant-related infections. The Ibis assay simultaneously tests for >3,000 species, including virtually all known pathogens, in a multiplex fashion, eliminating the need for an a priori choice regarding the likely infecting organism. For known pathogens, the Ibis is capable of yielding information down to the species level and can simultaneously assay for antibiotic resistance markers. It is semiquantitative and provides for a rapid turnaround, with clinically useful results available in as little as six hours. Although the Ibis T5000 is not yet approved by the Food and Drug Administration (FDA) for use in clinical diagnostics, we have used it to investigate clinical samples from multiple sources.
0021-9355
e851-e856
Gallo, Phillip H.
6b583441-aacb-48a4-9393-f8c9dcaaf84d
Melton-Kreft, Rachael
3a19cfa1-d348-4147-a252-5c54196421d4
Nistico, Laura
7a83886a-6bf1-46a1-87dd-75a120d41603
Sotereanos, Nicholas G.
826c42da-c093-4a32-a0e4-9a0e8b54912e
Sewecke, Jeffrey J.
64cfc387-097c-4c52-9286-93a317b452b5
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Ehrlich, Garth D.
aa8e5162-77a6-4627-a793-acd724ed0782
Costerton, J. William
3561239b-c96e-41af-9228-4fc120466c4b
Kathju, Sandeep
80cdb7ee-2e0d-4e70-98c9-93682ce05a09
Gallo, Phillip H.
6b583441-aacb-48a4-9393-f8c9dcaaf84d
Melton-Kreft, Rachael
3a19cfa1-d348-4147-a252-5c54196421d4
Nistico, Laura
7a83886a-6bf1-46a1-87dd-75a120d41603
Sotereanos, Nicholas G.
826c42da-c093-4a32-a0e4-9a0e8b54912e
Sewecke, Jeffrey J.
64cfc387-097c-4c52-9286-93a317b452b5
Stoodley, Paul
08614665-92a9-4466-806e-20c6daeb483f
Ehrlich, Garth D.
aa8e5162-77a6-4627-a793-acd724ed0782
Costerton, J. William
3561239b-c96e-41af-9228-4fc120466c4b
Kathju, Sandeep
80cdb7ee-2e0d-4e70-98c9-93682ce05a09

Gallo, Phillip H., Melton-Kreft, Rachael, Nistico, Laura, Sotereanos, Nicholas G., Sewecke, Jeffrey J., Stoodley, Paul, Ehrlich, Garth D., Costerton, J. William and Kathju, Sandeep (2011) Demonstration of bacillus cereus in orthopaedic-implant-related infection with use of a multi-primer polymerase chain reaction-mass spectrometric assay: report of two cases. The Journal of Bone and Joint Surgery, 93 (15), e851-e856. (doi:10.2106/JBJS.J.01181). (PMID:21915528)

Record type: Article

Abstract

The recent advent of a novel coupled PCR-mass spectrometric technology, the Ibis T5000(Abbott Laboratories, Chicago, Illinois) offers multiple potential advantages for molecular detection of periprosthetic joint infection or other implant-related infections. The Ibis assay simultaneously tests for >3,000 species, including virtually all known pathogens, in a multiplex fashion, eliminating the need for an a priori choice regarding the likely infecting organism. For known pathogens, the Ibis is capable of yielding information down to the species level and can simultaneously assay for antibiotic resistance markers. It is semiquantitative and provides for a rapid turnaround, with clinically useful results available in as little as six hours. Although the Ibis T5000 is not yet approved by the Food and Drug Administration (FDA) for use in clinical diagnostics, we have used it to investigate clinical samples from multiple sources.

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Published date: 3 August 2011
Organisations: nCATS Group

Identifiers

Local EPrints ID: 197415
URI: http://eprints.soton.ac.uk/id/eprint/197415
ISSN: 0021-9355
PURE UUID: 02127e52-3304-4242-81f6-4b396da747c3
ORCID for Paul Stoodley: ORCID iD orcid.org/0000-0001-6069-273X

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Date deposited: 23 Sep 2011 07:18
Last modified: 15 Mar 2024 03:34

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Contributors

Author: Phillip H. Gallo
Author: Rachael Melton-Kreft
Author: Laura Nistico
Author: Nicholas G. Sotereanos
Author: Jeffrey J. Sewecke
Author: Paul Stoodley ORCID iD
Author: Garth D. Ehrlich
Author: J. William Costerton
Author: Sandeep Kathju

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