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The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes

The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes
The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes
The immobilization of the mutants of L-lactate dehydrogenase (LDH) on poly(aniline) (PANi) composite films has been investigated. Mutants possessing peptide tags of varying charge and nucleophilicity were created to probe the nature of the interaction between the protein and PANi. These results are significant for the development of a 'generic' approach to the immobilization of enzymes and other proteins.
enzyme immobilization, bioanode, biofuel cell, cofactor recycling, nad(+), nicotinamide adenine-dinucleotide, lactate-dehydrogenase, poly(aniline)-coated electrodes, bacillus-stearothermophilus, oxidation, nadh
21-23
Halliwell, Catherine M.
6cc766e4-a027-4380-8604-c91a27301774
Simon, Evelyne
890fd27c-8885-4929-872f-0afc8d076526
Toh, Chee-Seng
271ecaf6-fbed-454e-aa92-c5e645ada23c
Cass, Anthony E.G.
6696e860-4ee0-42ea-958f-2cc9ca999e91
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Halliwell, Catherine M.
6cc766e4-a027-4380-8604-c91a27301774
Simon, Evelyne
890fd27c-8885-4929-872f-0afc8d076526
Toh, Chee-Seng
271ecaf6-fbed-454e-aa92-c5e645ada23c
Cass, Anthony E.G.
6696e860-4ee0-42ea-958f-2cc9ca999e91
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Halliwell, Catherine M., Simon, Evelyne, Toh, Chee-Seng, Cass, Anthony E.G. and Bartlett, Philip N. (2002) The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes. Bioelectrochemistry, 55 (1-2), 21-23. (doi:10.1016/S1567-5394(01)00172-4).

Record type: Article

Abstract

The immobilization of the mutants of L-lactate dehydrogenase (LDH) on poly(aniline) (PANi) composite films has been investigated. Mutants possessing peptide tags of varying charge and nucleophilicity were created to probe the nature of the interaction between the protein and PANi. These results are significant for the development of a 'generic' approach to the immobilization of enzymes and other proteins.

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More information

Published date: 2002
Keywords: enzyme immobilization, bioanode, biofuel cell, cofactor recycling, nad(+), nicotinamide adenine-dinucleotide, lactate-dehydrogenase, poly(aniline)-coated electrodes, bacillus-stearothermophilus, oxidation, nadh

Identifiers

Local EPrints ID: 19753
URI: http://eprints.soton.ac.uk/id/eprint/19753
PURE UUID: 067d2c0a-e758-430d-9c25-a40fd3e39998
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 20 Feb 2006
Last modified: 09 Jan 2022 02:42

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Contributors

Author: Catherine M. Halliwell
Author: Evelyne Simon
Author: Chee-Seng Toh
Author: Anthony E.G. Cass

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