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Determination of nitrofurantoin drug in pharmaceutical formulation and biological fluids by square-wave cathodic adsorptive stripping voltammetry

Determination of nitrofurantoin drug in pharmaceutical formulation and biological fluids by square-wave cathodic adsorptive stripping voltammetry
Determination of nitrofurantoin drug in pharmaceutical formulation and biological fluids by square-wave cathodic adsorptive stripping voltammetry
Nitrofurnation is an antibacterial drug. It is used in the treatment of initial or recurrent urinary tract infections caused by susceptible organisms. The cyclic voltammogram of the drug in Britton-Robinson buffers (pH 2-11) exhibited a single well-defined cathodic peak at the hanging mercury drop electrode, that due to the reduction of its nitro group to the amine stage. A fully validated, sensitive, and reproducible developed procedure was described for determination of the drug in bulk form, pharmaceutical formulation, human serum and human urine using, square-wave cathodic adsorptive stripping voltammetry. The optimal experimental parameters for the drug assay were: accumulation POTENTIAL=?0.4 V (vs. Ag/AgCl/ KCls), accumulation TIME=40 s, FREQUENCY=120 Hz, pulse AMPLITUDE=50 mV and scan INCREMENT=10 mV in Britton–Robinson buffer (pH 10). A mean percentage recovery of 100.68 +/- 0.17 (n = 5) and a detection limit of 1.32 x 10(-10) M of bulk drug were achieved. Applicability to assay of the drug in pharmaceutical formulation, human serum and human urine was studied and illustrated. The mean percentage recoveries were found as: 101.49 +/- 0.65, 103.94 +/- 0.73 and 101.98 +/- 0.52 (n = 5) in pharmaceutical formulation, human serum and human urine, respectively. Detection limits of 2.86 x 10(-10) M and 5.77 x 10(-10) M nitrofurantoin were achieved in human serum and urine, respectively.
nitrofurantoin determination, square-wave cathodic adsorptive stripping voltammetry, assay of pharmaceutical formulation, human serum and urine, liquid-chromatographic determination, glassy-carbon electrode, phenazopyridine, tablets, behavior, plasma, urine, feed
0731-7085
651-659
Hammam, Essam
6abf7e5d-5da6-4ce5-9948-109a1334770d
Hammam, Essam
6abf7e5d-5da6-4ce5-9948-109a1334770d

Hammam, Essam (2002) Determination of nitrofurantoin drug in pharmaceutical formulation and biological fluids by square-wave cathodic adsorptive stripping voltammetry. Journal of Pharmaceutical and Biomedical Analysis, 30 (3), 651-659. (doi:10.1016/S0731-7085(02)00344-8).

Record type: Article

Abstract

Nitrofurnation is an antibacterial drug. It is used in the treatment of initial or recurrent urinary tract infections caused by susceptible organisms. The cyclic voltammogram of the drug in Britton-Robinson buffers (pH 2-11) exhibited a single well-defined cathodic peak at the hanging mercury drop electrode, that due to the reduction of its nitro group to the amine stage. A fully validated, sensitive, and reproducible developed procedure was described for determination of the drug in bulk form, pharmaceutical formulation, human serum and human urine using, square-wave cathodic adsorptive stripping voltammetry. The optimal experimental parameters for the drug assay were: accumulation POTENTIAL=?0.4 V (vs. Ag/AgCl/ KCls), accumulation TIME=40 s, FREQUENCY=120 Hz, pulse AMPLITUDE=50 mV and scan INCREMENT=10 mV in Britton–Robinson buffer (pH 10). A mean percentage recovery of 100.68 +/- 0.17 (n = 5) and a detection limit of 1.32 x 10(-10) M of bulk drug were achieved. Applicability to assay of the drug in pharmaceutical formulation, human serum and human urine was studied and illustrated. The mean percentage recoveries were found as: 101.49 +/- 0.65, 103.94 +/- 0.73 and 101.98 +/- 0.52 (n = 5) in pharmaceutical formulation, human serum and human urine, respectively. Detection limits of 2.86 x 10(-10) M and 5.77 x 10(-10) M nitrofurantoin were achieved in human serum and urine, respectively.

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More information

Published date: 15 October 2002
Keywords: nitrofurantoin determination, square-wave cathodic adsorptive stripping voltammetry, assay of pharmaceutical formulation, human serum and urine, liquid-chromatographic determination, glassy-carbon electrode, phenazopyridine, tablets, behavior, plasma, urine, feed

Identifiers

Local EPrints ID: 19756
URI: http://eprints.soton.ac.uk/id/eprint/19756
ISSN: 0731-7085
PURE UUID: 60e624b3-ca0e-406d-a4d7-505e36dad52c

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Date deposited: 20 Feb 2006
Last modified: 08 Jan 2022 09:50

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Author: Essam Hammam

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