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Enzyme accessibility and solid supports: which molecular weight enzymes can be used on solid supports? An investigation using confocal Raman microscopy

Enzyme accessibility and solid supports: which molecular weight enzymes can be used on solid supports? An investigation using confocal Raman microscopy
Enzyme accessibility and solid supports: which molecular weight enzymes can be used on solid supports? An investigation using confocal Raman microscopy
The accessibility of various solid supports (TentaGel, PEGA 1900, and beaded controlled pore glasses (CPGs)) to a range of enzymes was investigated. The different beaded materials were loaded with the peptide 4-cyanobenzamide-Gly-Pro-Leu-Gly-Leu-Phe-Ala-Arg-OH and incubated with the enzymes MMP-12 (22 kDa), thermolysin, (35 kDa), MMP-13 (42.5 kDa), clostridium collagenase (68 kDa), and NEP (90 kDa). The absence/presence of the cyano stretching frequency was measured by means of confocal Raman microscopy. It was found that none of the investigated enzymes could enter the polymer matrices of TentaGel. PEGA 1900 was compatible only with the two smallest enzymes, while beaded CPG was successful even with NEP (90 kDa), proving its superiority over other materials in terms of bio-compatibility.
combinatorial chemistry, enzymes, raman spectroscopy, solid supports, combinatorial libraries, polymeric support, organic-synthesis, peptide-synthesis, linker, specificity, chemistry, beads, generation, resin
0009-305X
3769-3772
Kress, Jürgen
2a20514a-6783-43d3-9301-b6e7a7fbe3e9
Zanaletti, Riccardo
9c420ff6-8563-4cfd-97dc-2a8f7633ca75
Amour, Augustin
1cbf3c50-b09e-40e5-a1b2-f7f039e26eb9
Ladlow, Mark
9b8816c5-3cc6-462c-ac81-042dc167db53
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Kress, Jürgen
2a20514a-6783-43d3-9301-b6e7a7fbe3e9
Zanaletti, Riccardo
9c420ff6-8563-4cfd-97dc-2a8f7633ca75
Amour, Augustin
1cbf3c50-b09e-40e5-a1b2-f7f039e26eb9
Ladlow, Mark
9b8816c5-3cc6-462c-ac81-042dc167db53
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900

Kress, Jürgen, Zanaletti, Riccardo, Amour, Augustin, Ladlow, Mark, Frey, Jeremy G. and Bradley, Mark (2002) Enzyme accessibility and solid supports: which molecular weight enzymes can be used on solid supports? An investigation using confocal Raman microscopy. Chemistry, 8 (16), 3769-3772. (doi:10.1002/1521-3765(20020816)8:16<3769::AID-CHEM3769>3.0.CO;2-V).

Record type: Article

Abstract

The accessibility of various solid supports (TentaGel, PEGA 1900, and beaded controlled pore glasses (CPGs)) to a range of enzymes was investigated. The different beaded materials were loaded with the peptide 4-cyanobenzamide-Gly-Pro-Leu-Gly-Leu-Phe-Ala-Arg-OH and incubated with the enzymes MMP-12 (22 kDa), thermolysin, (35 kDa), MMP-13 (42.5 kDa), clostridium collagenase (68 kDa), and NEP (90 kDa). The absence/presence of the cyano stretching frequency was measured by means of confocal Raman microscopy. It was found that none of the investigated enzymes could enter the polymer matrices of TentaGel. PEGA 1900 was compatible only with the two smallest enzymes, while beaded CPG was successful even with NEP (90 kDa), proving its superiority over other materials in terms of bio-compatibility.

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More information

Published date: 2002
Keywords: combinatorial chemistry, enzymes, raman spectroscopy, solid supports, combinatorial libraries, polymeric support, organic-synthesis, peptide-synthesis, linker, specificity, chemistry, beads, generation, resin

Identifiers

Local EPrints ID: 19790
URI: http://eprints.soton.ac.uk/id/eprint/19790
ISSN: 0009-305X
PURE UUID: 1c549be6-b4e1-4822-a6d7-898df8aa07a5
ORCID for Jeremy G. Frey: ORCID iD orcid.org/0000-0003-0842-4302

Catalogue record

Date deposited: 21 Feb 2006
Last modified: 09 Jan 2022 02:33

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Contributors

Author: Jürgen Kress
Author: Riccardo Zanaletti
Author: Augustin Amour
Author: Mark Ladlow
Author: Jeremy G. Frey ORCID iD
Author: Mark Bradley

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