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Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina

Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina
Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina
The unambiguous assignment of the nuclear magnetic resonance (NMR) signals of the (alpha-substituents of the haems in the tetrahaem cytochrome isolated from Shewanella frigidimarina NCIMB400, was made using a combination of homonuclear and heteronuclear experiments. The paramagnetic C-13 shifts of the nuclei directly bound to the porphyrin of each haem group were analysed in the framework of a model for the haern electronic structure. The analysis yields g-tensors for each haem, which allowed the assignment of some electron paramagnetic resonance (EPR) signals to specific haems, and the orientation of the magnetic axes relative to each haem to be established. The orientation of the axial ligands of the haems was determined semi-empirically from the NMR data, and the structural results were compared with those of the homologous tetrahaern cytochrome from Shewanella oneidensis MR-1 showing significant similarities between the two proteins.
heteronuclear nuclear magnetic resonance, haem electronic structure, paramagnetic shift, electron transfer, shewanella, heme electronic-structure, spin ferric porphyrins, cytochrome c(3), fumarate reductase, putrefaciens mr-1, susceptibility tensors, protontranslocation, nmr, shifts, proteins
0014-5793
520-524
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pessanha, Miguel
7e563109-5e0f-4949-b0cc-9cab7a8e2809
Reid, Graeme A.
ff22c1ca-6493-43e5-9def-d46ae425eaf7
Chapman, Stephen K.
c8004be1-78bc-406c-8150-2cc282f910fa
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Salgueiro, Carlos A.
a9805ac9-e610-42ca-bb85-ef4e288eeffb
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pessanha, Miguel
7e563109-5e0f-4949-b0cc-9cab7a8e2809
Reid, Graeme A.
ff22c1ca-6493-43e5-9def-d46ae425eaf7
Chapman, Stephen K.
c8004be1-78bc-406c-8150-2cc282f910fa
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Salgueiro, Carlos A.
a9805ac9-e610-42ca-bb85-ef4e288eeffb

Louro, Ricardo O., Pessanha, Miguel, Reid, Graeme A., Chapman, Stephen K., Turner, David L. and Salgueiro, Carlos A. (2002) Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina. FEBS Letters, 531 (3), 520-524. (doi:10.1016/S0014-5793(02)03610-4).

Record type: Article

Abstract

The unambiguous assignment of the nuclear magnetic resonance (NMR) signals of the (alpha-substituents of the haems in the tetrahaem cytochrome isolated from Shewanella frigidimarina NCIMB400, was made using a combination of homonuclear and heteronuclear experiments. The paramagnetic C-13 shifts of the nuclei directly bound to the porphyrin of each haem group were analysed in the framework of a model for the haern electronic structure. The analysis yields g-tensors for each haem, which allowed the assignment of some electron paramagnetic resonance (EPR) signals to specific haems, and the orientation of the magnetic axes relative to each haem to be established. The orientation of the axial ligands of the haems was determined semi-empirically from the NMR data, and the structural results were compared with those of the homologous tetrahaern cytochrome from Shewanella oneidensis MR-1 showing significant similarities between the two proteins.

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More information

Published date: 20 November 2002
Keywords: heteronuclear nuclear magnetic resonance, haem electronic structure, paramagnetic shift, electron transfer, shewanella, heme electronic-structure, spin ferric porphyrins, cytochrome c(3), fumarate reductase, putrefaciens mr-1, susceptibility tensors, protontranslocation, nmr, shifts, proteins

Identifiers

Local EPrints ID: 19809
URI: http://eprints.soton.ac.uk/id/eprint/19809
ISSN: 0014-5793
PURE UUID: 74d69efc-52a1-4924-b5de-9552f90aa644

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Date deposited: 21 Feb 2006
Last modified: 08 Jan 2022 01:00

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Contributors

Author: Ricardo O. Louro
Author: Miguel Pessanha
Author: Graeme A. Reid
Author: Stephen K. Chapman
Author: David L. Turner
Author: Carlos A. Salgueiro

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