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Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli

Record type: Article

Biotin synthase (BioB) catalyzes the insertion of a sulfur atom between the C6 and C9 carbons of dethiobiotin. Reconstituted BioB from Escherichia coli contains a [4Fe-4S](2+/1+) cluster thought to be involved in the reduction and cleavage of S-adenosylmethionine (AdoMet), generating methionine and the reactive 5'-deoxyadenosyl radical responsible for dethiobiotin H-abstraction. Using EPR and Mössbauer spectroscopy as well as methionine quantitation we demonstrate that the reduced S = 1/2 [4Fe-4S](1+) cluster is indeed capable of injecting one electron into AdoMet, generating one equivalent of both methionine and S = 0 [4Fe-4S](2+) cluster. Dethiobiotin is not required for the reaction. Using site-directed mutagenesis we show also that, among the eight cysteines of BioB, only three (Cys-53, Cys-57, Cys-60) are essential for AdoMet reductive cleavage, suggesting that these cysteines are involved in chelation of the [4Fe-4S](2+/1+) cluster.

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Citation

Ollagnier-de Choudens, Sandrine, Sanakis, Yiannis, Hewitson, Kirsty S., Roach, Peter, Münck, Eckard and Fontecave, Marc (2002) Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli The Journal of Biological Chemistry, 277, (16), pp. 13449-13454. (doi:10.1074/jbc.M111324200).

More information

Published date: 19 April 2002
Keywords: iron-sulfur cluster, pyruvate formate-lyase, anaerobic ribonucleotide reductase, electron-paramagnetic-resonance, lysine 2, 3-aminomutase, radical mechanisms, activating enzyme, single turnover, in-vitro, protein

Identifiers

Local EPrints ID: 19832
URI: http://eprints.soton.ac.uk/id/eprint/19832
ISSN: 0021-9258
PURE UUID: 6bd66560-1702-4fa9-9d0a-ff328994910c

Catalogue record

Date deposited: 22 Feb 2006
Last modified: 17 Jul 2017 16:30

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Contributors

Author: Sandrine Ollagnier-de Choudens
Author: Yiannis Sanakis
Author: Kirsty S. Hewitson
Author: Peter Roach
Author: Eckard Münck
Author: Marc Fontecave

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