Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774
Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774
Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pK(a)(ox) and PK(a)red values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.
nine-haem cytochome c, redox-bohr effect, energy transduction, electrostatic models, desulfovibrio desulfuricans, redox titrations, vulgaris hildenborough, electron-transfer, primarysequence, c(3), cooperativity, atcc-27774, thermodynamics, protons, complex
248-252
Reis, Clara
44ab0e8c-f627-4a26-86cd-a1bdebf7f584
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pacheco, Isabel
c8de86d6-fab8-476c-8e1c-ebe3c34902b8
Catarino, Teresa
608a4400-1611-4798-bf27-7472e7c6a476
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Xavier, António V.
77269a6a-27cb-4c75-abf0-7d03ac64c228
15 November 2002
Reis, Clara
44ab0e8c-f627-4a26-86cd-a1bdebf7f584
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pacheco, Isabel
c8de86d6-fab8-476c-8e1c-ebe3c34902b8
Catarino, Teresa
608a4400-1611-4798-bf27-7472e7c6a476
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Xavier, António V.
77269a6a-27cb-4c75-abf0-7d03ac64c228
Reis, Clara, Louro, Ricardo O., Pacheco, Isabel, Catarino, Teresa, Turner, David L. and Xavier, António V.
(2002)
Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774.
Inorganica Chimica Acta, 339, .
(doi:10.1016/S0020-1693(02)00951-9).
Abstract
Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pK(a)(ox) and PK(a)red values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.
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Published date: 15 November 2002
Keywords:
nine-haem cytochome c, redox-bohr effect, energy transduction, electrostatic models, desulfovibrio desulfuricans, redox titrations, vulgaris hildenborough, electron-transfer, primarysequence, c(3), cooperativity, atcc-27774, thermodynamics, protons, complex
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Local EPrints ID: 19842
URI: http://eprints.soton.ac.uk/id/eprint/19842
ISSN: 0020-1693
PURE UUID: 743e9f7c-f26b-4f93-9e58-7e03c0a25b48
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Date deposited: 22 Feb 2006
Last modified: 15 Mar 2024 06:19
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Author:
Clara Reis
Author:
Ricardo O. Louro
Author:
Isabel Pacheco
Author:
Teresa Catarino
Author:
David L. Turner
Author:
António V. Xavier
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