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Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774

Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774
Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774
Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pK(a)(ox) and PK(a)red values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.
nine-haem cytochome c, redox-bohr effect, energy transduction, electrostatic models, desulfovibrio desulfuricans, redox titrations, vulgaris hildenborough, electron-transfer, primarysequence, c(3), cooperativity, atcc-27774, thermodynamics, protons, complex
0020-1693
248-252
Reis, Clara
44ab0e8c-f627-4a26-86cd-a1bdebf7f584
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pacheco, Isabel
c8de86d6-fab8-476c-8e1c-ebe3c34902b8
Catarino, Teresa
608a4400-1611-4798-bf27-7472e7c6a476
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Xavier, António V.
77269a6a-27cb-4c75-abf0-7d03ac64c228
Reis, Clara
44ab0e8c-f627-4a26-86cd-a1bdebf7f584
Louro, Ricardo O.
8014cf88-eb58-4ecd-8e04-8ce90a4e43f6
Pacheco, Isabel
c8de86d6-fab8-476c-8e1c-ebe3c34902b8
Catarino, Teresa
608a4400-1611-4798-bf27-7472e7c6a476
Turner, David L.
6c4c1ccb-a6f2-47cf-944e-e69269198a29
Xavier, António V.
77269a6a-27cb-4c75-abf0-7d03ac64c228

Reis, Clara, Louro, Ricardo O., Pacheco, Isabel, Catarino, Teresa, Turner, David L. and Xavier, António V. (2002) Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774. Inorganica Chimica Acta, 339, 248-252. (doi:10.1016/S0020-1693(02)00951-9).

Record type: Article

Abstract

Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pK(a)(ox) and PK(a)red values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.

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More information

Published date: 15 November 2002
Keywords: nine-haem cytochome c, redox-bohr effect, energy transduction, electrostatic models, desulfovibrio desulfuricans, redox titrations, vulgaris hildenborough, electron-transfer, primarysequence, c(3), cooperativity, atcc-27774, thermodynamics, protons, complex

Identifiers

Local EPrints ID: 19842
URI: http://eprints.soton.ac.uk/id/eprint/19842
ISSN: 0020-1693
PURE UUID: 743e9f7c-f26b-4f93-9e58-7e03c0a25b48

Catalogue record

Date deposited: 22 Feb 2006
Last modified: 08 Jan 2022 06:46

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Contributors

Author: Clara Reis
Author: Ricardo O. Louro
Author: Isabel Pacheco
Author: Teresa Catarino
Author: David L. Turner
Author: António V. Xavier

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