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Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications

Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications
Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications
Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)-poly(anion) composite films, PANi-poly(vinylsulfonate) (PVS) or PANi-poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of ?-nicotinamide adenine dinucleotide (NAD+) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 °C.
enzyme immobilisation, poly(aniline) composite films, nadh, biofuel cell, lactate-dehydrogenase, polyaniline films, immobilisation, electrodes, oxidation
13-15
Simon, Evelyne
890fd27c-8885-4929-872f-0afc8d076526
Halliwell, Catherine M.
6cc766e4-a027-4380-8604-c91a27301774
Toh, Chee Seng
87a356a3-63ee-41f6-8aab-84813ad8e30e
Cass, Anthony E.G.
6696e860-4ee0-42ea-958f-2cc9ca999e91
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Simon, Evelyne
890fd27c-8885-4929-872f-0afc8d076526
Halliwell, Catherine M.
6cc766e4-a027-4380-8604-c91a27301774
Toh, Chee Seng
87a356a3-63ee-41f6-8aab-84813ad8e30e
Cass, Anthony E.G.
6696e860-4ee0-42ea-958f-2cc9ca999e91
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Simon, Evelyne, Halliwell, Catherine M., Toh, Chee Seng, Cass, Anthony E.G. and Bartlett, Philip N. (2002) Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications. Bioelectrochemistry, 55 (1-2), 13-15. (doi:10.1016/S1567-5394(01)00160-8).

Record type: Article

Abstract

Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)-poly(anion) composite films, PANi-poly(vinylsulfonate) (PVS) or PANi-poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of ?-nicotinamide adenine dinucleotide (NAD+) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 °C.

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More information

Published date: 2002
Keywords: enzyme immobilisation, poly(aniline) composite films, nadh, biofuel cell, lactate-dehydrogenase, polyaniline films, immobilisation, electrodes, oxidation

Identifiers

Local EPrints ID: 19854
URI: http://eprints.soton.ac.uk/id/eprint/19854
PURE UUID: fe220eb4-5325-4d06-a2cb-bb31c61c563b
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 22 Feb 2006
Last modified: 08 Jan 2022 02:38

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Contributors

Author: Evelyne Simon
Author: Catherine M. Halliwell
Author: Chee Seng Toh
Author: Anthony E.G. Cass

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