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Novel photocleavable universal support for oligonucleotide synthesis

Novel photocleavable universal support for oligonucleotide synthesis
Novel photocleavable universal support for oligonucleotide synthesis
A novel photocleavable universal support for the automated solid phase synthesis of oligonucleotides is described. The linker between the growing oligonucleotide chain and CPG support contains a nucleophilic amine protected with a photocleavable group. On exposure to UV light, this group is detached and the free amine affords cleavage of the oligonucleotide from the support. The use of long wavelength UV light avoids damage to the DNA.
photocleavable support, universal support, oligonucleotide synthesis
1525-7770
1403-1406
Anderson, Emma
374e6b5f-424b-4649-af32-0fbcb4d353fa
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Picken, Douglas
878fbe4f-de3c-4e16-acd1-0803721d8c12
Anderson, Emma
374e6b5f-424b-4649-af32-0fbcb4d353fa
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Picken, Douglas
878fbe4f-de3c-4e16-acd1-0803721d8c12

Anderson, Emma, Brown, Tom and Picken, Douglas (2003) Novel photocleavable universal support for oligonucleotide synthesis. Nucleosides, Nucleotides & Nucleic Acids, 22 (5-8), 1403-1406.

Record type: Article

Abstract

A novel photocleavable universal support for the automated solid phase synthesis of oligonucleotides is described. The linker between the growing oligonucleotide chain and CPG support contains a nucleophilic amine protected with a photocleavable group. On exposure to UV light, this group is detached and the free amine affords cleavage of the oligonucleotide from the support. The use of long wavelength UV light avoids damage to the DNA.

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More information

Published date: 2003
Keywords: photocleavable support, universal support, oligonucleotide synthesis

Identifiers

Local EPrints ID: 19880
URI: http://eprints.soton.ac.uk/id/eprint/19880
ISSN: 1525-7770
PURE UUID: e42a0e15-7abc-4f55-90ef-1f7d2ab23388

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Date deposited: 23 Feb 2006
Last modified: 27 Apr 2022 04:19

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Contributors

Author: Emma Anderson
Author: Tom Brown
Author: Douglas Picken

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