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Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode

Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode
Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode
Kinetic isotope measurements using [4,4-2H2]NADH and [4-1H, 4-2H]NADH have been used to investigate the mechanism of the electrochemical oxidation of NADH at poly(aniline)-poly(vinyl sulfonate)-modified electrodes. The experiments show a primary kinetic isotope effect for the reaction of 4.2. This is consistent with literature values for the corresponding isotope effect for the oxidation of NADH by two-electron oxidants in homogeneous solution. The result demonstrates that transfer of H from NADH to the modified electrode occurs in the rate-limiting step within the reaction complex.
nicotinamide adenine-dinucleotide, hydride transfer, analogs
0002-7863
4014-4015
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Simon, Evelyne
890fd27c-8885-4929-872f-0afc8d076526
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Simon, Evelyne
890fd27c-8885-4929-872f-0afc8d076526

Bartlett, Philip N. and Simon, Evelyne (2003) Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode. Journal of the American Chemical Society, 125 (14), 4014-4015. (doi:10.1021/ja028943e).

Record type: Article

Abstract

Kinetic isotope measurements using [4,4-2H2]NADH and [4-1H, 4-2H]NADH have been used to investigate the mechanism of the electrochemical oxidation of NADH at poly(aniline)-poly(vinyl sulfonate)-modified electrodes. The experiments show a primary kinetic isotope effect for the reaction of 4.2. This is consistent with literature values for the corresponding isotope effect for the oxidation of NADH by two-electron oxidants in homogeneous solution. The result demonstrates that transfer of H from NADH to the modified electrode occurs in the rate-limiting step within the reaction complex.

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More information

Published date: 9 April 2003
Keywords: nicotinamide adenine-dinucleotide, hydride transfer, analogs

Identifiers

Local EPrints ID: 19892
URI: https://eprints.soton.ac.uk/id/eprint/19892
ISSN: 0002-7863
PURE UUID: 3b9257f6-f505-4c79-98f6-98ec77e1d5c5
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 23 Feb 2006
Last modified: 17 Jul 2019 19:03

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