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Generation of chemical movies: FT-IR spectroscopic imaging of segmented flows

Generation of chemical movies: FT-IR spectroscopic imaging of segmented flows
Generation of chemical movies: FT-IR spectroscopic imaging of segmented flows
We have previously demonstrated that FT-IR spectroscopic imaging can be used as a powerful, label-free detection method for studying laminar flows. However, to date, the speed of image acquisition has been too slow for the efficient detection of moving droplets within segmented flow systems. In this paper, we demonstrate the extraction of fast FT-IR images with acquisition times of 50 ms. This approach allows efficient interrogation of segmented flow systems where aqueous droplets move at a speed of 2.5 mm/s. Consecutive FT-IR images separated by 120 ms intervals allow the generation of chemical movies at eight frames per second. The technique has been applied to the study of microfluidic systems containing moving droplets of water in oil and droplets of protein solution in oil. The presented work demonstrates the feasibility of the use of FT-IR imaging to study dynamic systems with subsecond temporal resolution.
0003-2700
3606-3609
Chan, K.L. Andrew
0d7bc0b4-b955-4bd5-bfda-ff541a4e69fa
Niu, X.
f3d964fb-23b4-45db-92fe-02426e4e76fa
deMello, A.J.
ad9b4842-b673-4c9c-8d6a-0ed637fb7755
Kazarian, S.G.
7f61e494-3860-4ae7-9454-7660aa25c075
Chan, K.L. Andrew
0d7bc0b4-b955-4bd5-bfda-ff541a4e69fa
Niu, X.
f3d964fb-23b4-45db-92fe-02426e4e76fa
deMello, A.J.
ad9b4842-b673-4c9c-8d6a-0ed637fb7755
Kazarian, S.G.
7f61e494-3860-4ae7-9454-7660aa25c075

Chan, K.L. Andrew, Niu, X., deMello, A.J. and Kazarian, S.G. (2011) Generation of chemical movies: FT-IR spectroscopic imaging of segmented flows. Analytical Chemistry, 83 (9), 3606-3609. (doi:10.1021/ac200497a).

Record type: Article

Abstract

We have previously demonstrated that FT-IR spectroscopic imaging can be used as a powerful, label-free detection method for studying laminar flows. However, to date, the speed of image acquisition has been too slow for the efficient detection of moving droplets within segmented flow systems. In this paper, we demonstrate the extraction of fast FT-IR images with acquisition times of 50 ms. This approach allows efficient interrogation of segmented flow systems where aqueous droplets move at a speed of 2.5 mm/s. Consecutive FT-IR images separated by 120 ms intervals allow the generation of chemical movies at eight frames per second. The technique has been applied to the study of microfluidic systems containing moving droplets of water in oil and droplets of protein solution in oil. The presented work demonstrates the feasibility of the use of FT-IR imaging to study dynamic systems with subsecond temporal resolution.

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More information

e-pub ahead of print date: 26 March 2011
Published date: 2011
Organisations: Mechatronics

Identifiers

Local EPrints ID: 199877
URI: https://eprints.soton.ac.uk/id/eprint/199877
ISSN: 0003-2700
PURE UUID: 95083d2f-14ba-431b-abc1-d9745e61619e

Catalogue record

Date deposited: 21 Oct 2011 13:58
Last modified: 18 Jul 2017 11:15

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