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Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed

Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed
Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed
The electrokinetic transport behavior of ?-DNA (48?kbp) in 20?nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP (‘nano-gel’) can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
?-dna, biased reptation, caterpillar movement, nanoslits, pathways, pvp
0173-0835
2402-2409
Castillo-Fernandez, Oscar
3b88aa60-ab90-4a85-bde2-4f380d75ccbf
Salieb-Beugelaar, Georgette B.
62a65a50-be5d-48fd-b273-7a1f78de16e7
Bomer, Johan G.
dd026567-bd3d-46ad-a6bd-893412aada88
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Samitier, Josep
7735286e-85a5-4b4d-b8af-2826e1441b77
van den Berg, Albert
3cd94d32-26da-41a1-8505-18b7cd3edcbd
Eijkel, Jan C.T.
52cfa014-54ac-42b1-ad97-d1221c560f9f
Castillo-Fernandez, Oscar
3b88aa60-ab90-4a85-bde2-4f380d75ccbf
Salieb-Beugelaar, Georgette B.
62a65a50-be5d-48fd-b273-7a1f78de16e7
Bomer, Johan G.
dd026567-bd3d-46ad-a6bd-893412aada88
Arundell, Martin
e24d3405-a436-4d65-a6e4-2939ede79678
Samitier, Josep
7735286e-85a5-4b4d-b8af-2826e1441b77
van den Berg, Albert
3cd94d32-26da-41a1-8505-18b7cd3edcbd
Eijkel, Jan C.T.
52cfa014-54ac-42b1-ad97-d1221c560f9f

Castillo-Fernandez, Oscar, Salieb-Beugelaar, Georgette B., Bomer, Johan G., Arundell, Martin, Samitier, Josep, van den Berg, Albert and Eijkel, Jan C.T. (2011) Electrokinetic DNA transport in 20?nm-high nanoslits: Evidence for movement through a wall-adsorbed. Electrophoresis, 32 (18), 2402-2409. (doi:10.1002/elps.201100278). (PMID:21922490)

Record type: Article

Abstract

The electrokinetic transport behavior of ?-DNA (48?kbp) in 20?nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP (‘nano-gel’) can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

Full text not available from this repository.

More information

e-pub ahead of print date: 26 August 2011
Published date: September 2011
Keywords: ?-dna, biased reptation, caterpillar movement, nanoslits, pathways, pvp
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 200275
URI: https://eprints.soton.ac.uk/id/eprint/200275
ISSN: 0173-0835
PURE UUID: 69a95a66-7a54-413a-b097-8c370d9b2c5b

Catalogue record

Date deposited: 24 Oct 2011 16:01
Last modified: 16 Jul 2019 23:21

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