A new dark quencher for use in genetic analysis
A new dark quencher for use in genetic analysis
A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.
scorpion primers, fluorescence
970-971
May, JP
b54a262b-9f32-4435-8866-3b9c495294f3
Brown, LJ
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
Rudloff, I
c7ced4b7-c466-49ea-bcae-f1b37613845a
Brown, T
a64aae36-bb30-42df-88a2-11be394e8c89
2003
May, JP
b54a262b-9f32-4435-8866-3b9c495294f3
Brown, LJ
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
Rudloff, I
c7ced4b7-c466-49ea-bcae-f1b37613845a
Brown, T
a64aae36-bb30-42df-88a2-11be394e8c89
May, JP, Brown, LJ, Rudloff, I and Brown, T
(2003)
A new dark quencher for use in genetic analysis.
Chemical Communications, (8), .
(doi:10.1039/b300934c).
Abstract
A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.
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Published date: 2003
Keywords:
scorpion primers, fluorescence
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Local EPrints ID: 20044
URI: http://eprints.soton.ac.uk/id/eprint/20044
ISSN: 1359-7345
PURE UUID: c593f56a-3002-4669-8239-7f41321b4d50
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Date deposited: 22 Feb 2006
Last modified: 16 Mar 2024 04:38
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Author:
I Rudloff
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