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A new dark quencher for use in genetic analysis

May, J.P., Brown, L.J., Rudloff, I. and Brown, T. (2003) A new dark quencher for use in genetic analysis Chemical Communications, (8), pp. 970-971. (doi:10.1039/b300934c).

Record type: Article

Abstract

A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.

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Published date: 2003
Keywords: scorpion primers, fluorescence

Identifiers

Local EPrints ID: 20044
URI: http://eprints.soton.ac.uk/id/eprint/20044
ISSN: 1359-7345
PURE UUID: c593f56a-3002-4669-8239-7f41321b4d50

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Date deposited: 22 Feb 2006
Last modified: 17 Jul 2017 16:29

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Contributors

Author: J.P. May
Author: L.J. Brown
Author: I. Rudloff
Author: T. Brown

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