Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes
Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes
We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.
fluorescence energy-transfer, molecular beacons, dna triplex, oligonucleotides, duplex, hybridization
2267-2275
McKeen, Catherine M.
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Brown, Lynda J.
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
Nicol, Jamie T.G.
eb81f29d-a3e0-47d9-bec2-1f254ee372c4
Mellor, John M.
116e0a09-9f67-4a1e-8110-9a6f27772ed3
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
2003
McKeen, Catherine M.
44153a47-668b-4cfa-808c-15552c9f74d8
Brown, Lynda J.
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
Nicol, Jamie T.G.
eb81f29d-a3e0-47d9-bec2-1f254ee372c4
Mellor, John M.
116e0a09-9f67-4a1e-8110-9a6f27772ed3
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
McKeen, Catherine M., Brown, Lynda J., Nicol, Jamie T.G., Mellor, John M. and Brown, Tom
(2003)
Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes.
Organic & Biomolecular Chemistry, 1 (13), .
(doi:10.1039/b301859h).
(PMID:12945696)
Abstract
We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.
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Published date: 2003
Keywords:
fluorescence energy-transfer, molecular beacons, dna triplex, oligonucleotides, duplex, hybridization
Identifiers
Local EPrints ID: 20047
URI: http://eprints.soton.ac.uk/id/eprint/20047
ISSN: 1477-0520
PURE UUID: d96aeaf1-9644-4781-bee1-805597a1fe44
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Date deposited: 24 Feb 2006
Last modified: 16 Mar 2024 03:00
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Contributors
Author:
Catherine M. McKeen
Author:
Jamie T.G. Nicol
Author:
John M. Mellor
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