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Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes

Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes
Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes
We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.
fluorescence energy-transfer, molecular beacons, dna triplex, oligonucleotides, duplex, hybridization
1477-0520
2267-2275
McKeen, Catherine M.
44153a47-668b-4cfa-808c-15552c9f74d8
Brown, Lynda J.
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
Nicol, Jamie T.G.
eb81f29d-a3e0-47d9-bec2-1f254ee372c4
Mellor, John M.
116e0a09-9f67-4a1e-8110-9a6f27772ed3
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
McKeen, Catherine M.
44153a47-668b-4cfa-808c-15552c9f74d8
Brown, Lynda J.
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
Nicol, Jamie T.G.
eb81f29d-a3e0-47d9-bec2-1f254ee372c4
Mellor, John M.
116e0a09-9f67-4a1e-8110-9a6f27772ed3
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

McKeen, Catherine M., Brown, Lynda J., Nicol, Jamie T.G., Mellor, John M. and Brown, Tom (2003) Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes. Organic & Biomolecular Chemistry, 1 (13), 2267-2275. (doi:10.1039/b301859h). (PMID:12945696)

Record type: Article

Abstract

We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.

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More information

Published date: 2003
Keywords: fluorescence energy-transfer, molecular beacons, dna triplex, oligonucleotides, duplex, hybridization

Identifiers

Local EPrints ID: 20047
URI: http://eprints.soton.ac.uk/id/eprint/20047
ISSN: 1477-0520
PURE UUID: d96aeaf1-9644-4781-bee1-805597a1fe44
ORCID for Lynda J. Brown: ORCID iD orcid.org/0000-0002-5678-0814

Catalogue record

Date deposited: 24 Feb 2006
Last modified: 16 Mar 2024 03:00

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Contributors

Author: Catherine M. McKeen
Author: Lynda J. Brown ORCID iD
Author: Jamie T.G. Nicol
Author: John M. Mellor
Author: Tom Brown

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