The University of Southampton
University of Southampton Institutional Repository

pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts

Record type: Article

The properties of membrane-embedded GTPases are investigated to understand translocation of preprotein across the outer envelope of chloroplasts. The homo- and heterodimerization events of the GTPases had been established previously. We show that the hydrolytic activity of the GTPase Toc33 is pH insensitive in the homodimeric conformation but has a bell-shaped pH optimum in the monomeric conformation. Further, Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive as well. pH sensitivity might serve to regulate translocation; alternatively, the documented pH sensitivity might reflect a mechanistic requirement for GTPase silencing during translocation as the GTPase switches between homo- and heterodimeric conformations.

PDF PlantCellPhysiol_49-1917.pdf - Version of Record
Restricted to Repository staff only
Download (167kB)

Citation

Bionda, Tihana, Koenig, Patrick, Oreb, Mislav, Tews, Ivo and Schleiff, Enrico (2008) pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts Plant and Cell Physiology, 49, (12), pp. 1917-1921. (doi:10.1093/pcp/pcn171). (PMID:19001421).

More information

e-pub ahead of print date: 10 November 2008
Published date: December 2008
Keywords: arabidopsis thaliana and Pisum sativum, imerization, gtpase, ph sensitivity, protein translocation, toc
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 200515
URI: http://eprints.soton.ac.uk/id/eprint/200515
ISSN: 0032-0781
PURE UUID: 96726927-32fb-47f8-99f2-10678197b6ce
ORCID for Ivo Tews: ORCID iD orcid.org/0000-0002-4704-1139

Catalogue record

Date deposited: 26 Oct 2011 10:40
Last modified: 18 Jul 2017 11:14

Export record

Altmetrics

Contributors

Author: Tihana Bionda
Author: Patrick Koenig
Author: Mislav Oreb
Author: Ivo Tews ORCID iD
Author: Enrico Schleiff

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×