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pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts

pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts
pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts
The properties of membrane-embedded GTPases are investigated to understand translocation of preprotein across the outer envelope of chloroplasts. The homo- and heterodimerization events of the GTPases had been established previously. We show that the hydrolytic activity of the GTPase Toc33 is pH insensitive in the homodimeric conformation but has a bell-shaped pH optimum in the monomeric conformation. Further, Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive as well. pH sensitivity might serve to regulate translocation; alternatively, the documented pH sensitivity might reflect a mechanistic requirement for GTPase silencing during translocation as the GTPase switches between homo- and heterodimeric conformations.
arabidopsis thaliana and Pisum sativum, imerization, gtpase, ph sensitivity, protein translocation, toc
0032-0781
1917-1921
Bionda, Tihana
d3a07ba5-d51b-4eb9-8e04-88cfc52ac3f6
Koenig, Patrick
e03428c5-67ef-4df4-9310-77190d216517
Oreb, Mislav
85bcf606-2b7c-4362-a88f-b99bf6cbe748
Tews, Ivo
9117fc5e-d01c-4f8d-a734-5b14d3eee8dd
Schleiff, Enrico
119114bb-9cbb-45e9-a161-db45b1e9f4ba
Bionda, Tihana
d3a07ba5-d51b-4eb9-8e04-88cfc52ac3f6
Koenig, Patrick
e03428c5-67ef-4df4-9310-77190d216517
Oreb, Mislav
85bcf606-2b7c-4362-a88f-b99bf6cbe748
Tews, Ivo
9117fc5e-d01c-4f8d-a734-5b14d3eee8dd
Schleiff, Enrico
119114bb-9cbb-45e9-a161-db45b1e9f4ba

Bionda, Tihana, Koenig, Patrick, Oreb, Mislav, Tews, Ivo and Schleiff, Enrico (2008) pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts. Plant and Cell Physiology, 49 (12), 1917-1921. (doi:10.1093/pcp/pcn171). (PMID:19001421)

Record type: Article

Abstract

The properties of membrane-embedded GTPases are investigated to understand translocation of preprotein across the outer envelope of chloroplasts. The homo- and heterodimerization events of the GTPases had been established previously. We show that the hydrolytic activity of the GTPase Toc33 is pH insensitive in the homodimeric conformation but has a bell-shaped pH optimum in the monomeric conformation. Further, Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive as well. pH sensitivity might serve to regulate translocation; alternatively, the documented pH sensitivity might reflect a mechanistic requirement for GTPase silencing during translocation as the GTPase switches between homo- and heterodimeric conformations.

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e-pub ahead of print date: 10 November 2008
Published date: December 2008
Keywords: arabidopsis thaliana and Pisum sativum, imerization, gtpase, ph sensitivity, protein translocation, toc
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 200515
URI: https://eprints.soton.ac.uk/id/eprint/200515
ISSN: 0032-0781
PURE UUID: 96726927-32fb-47f8-99f2-10678197b6ce
ORCID for Ivo Tews: ORCID iD orcid.org/0000-0002-4704-1139

Catalogue record

Date deposited: 26 Oct 2011 10:40
Last modified: 06 Jun 2018 12:33

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