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Cell surface counter receptors are essential components of the unconventional export machinery of galectin-1

Cell surface counter receptors are essential components of the unconventional export machinery of galectin-1
Cell surface counter receptors are essential components of the unconventional export machinery of galectin-1
Galectin-1 is a component of the extracellular matrix as well as a ligand of cell surface counter receptors such as beta-galactoside-containing glycolipids, however, the molecular mechanism of galectin-1 secretion has remained elusive. Based on a nonbiased screen for galectin-1 export mutants we have identified 26 single amino acid changes that cause a defect of both export and binding to counter receptors. When wild-type galectin-1 was analyzed in CHO clone 13 cells, a mutant cell line incapable of expressing functional galectin-1 counter receptors, secretion was blocked. Intriguingly, we also find that a distant relative of galectin-1, the fungal lectin CGL-2, is a substrate for nonclassical export from Chinese hamster ovary (CHO) cells. Alike mammalian galectin-1, a CGL-2 mutant defective in beta-galactoside binding, does not get exported from CHO cells. We conclude that the beta-galactoside binding site represents the primary targeting motif of galectins defining a galectin export machinery that makes use of beta-galactoside-containing surface molecules as export receptors for intracellular galectin-1.
1932-6203
373-381
Seelenmeyer, Claudia
1962ef7b-38af-4a5b-b90a-4cfd5a4bf7c5
Wegehingel, Sabine
6a0766a6-4a34-45e3-b4a1-92aba7ae7bd2
Tews, Ivo
9117fc5e-d01c-4f8d-a734-5b14d3eee8dd
Künzler, Markus
20d36c1c-a24b-4bcf-9c26-8bdd5525f03b
Aebi, Markus
0b06ade4-206e-4f8b-96c2-5146d93f8a86
Nickel, Walter
ccabdc92-e871-48e1-9960-5164e8587513
Seelenmeyer, Claudia
1962ef7b-38af-4a5b-b90a-4cfd5a4bf7c5
Wegehingel, Sabine
6a0766a6-4a34-45e3-b4a1-92aba7ae7bd2
Tews, Ivo
9117fc5e-d01c-4f8d-a734-5b14d3eee8dd
Künzler, Markus
20d36c1c-a24b-4bcf-9c26-8bdd5525f03b
Aebi, Markus
0b06ade4-206e-4f8b-96c2-5146d93f8a86
Nickel, Walter
ccabdc92-e871-48e1-9960-5164e8587513

Seelenmeyer, Claudia, Wegehingel, Sabine, Tews, Ivo, Künzler, Markus, Aebi, Markus and Nickel, Walter (2005) Cell surface counter receptors are essential components of the unconventional export machinery of galectin-1. PLoS ONE, 171 (2), 373-381. (doi:10.1083/jcb.200905083). (PMID:10931889)

Record type: Article

Abstract

Galectin-1 is a component of the extracellular matrix as well as a ligand of cell surface counter receptors such as beta-galactoside-containing glycolipids, however, the molecular mechanism of galectin-1 secretion has remained elusive. Based on a nonbiased screen for galectin-1 export mutants we have identified 26 single amino acid changes that cause a defect of both export and binding to counter receptors. When wild-type galectin-1 was analyzed in CHO clone 13 cells, a mutant cell line incapable of expressing functional galectin-1 counter receptors, secretion was blocked. Intriguingly, we also find that a distant relative of galectin-1, the fungal lectin CGL-2, is a substrate for nonclassical export from Chinese hamster ovary (CHO) cells. Alike mammalian galectin-1, a CGL-2 mutant defective in beta-galactoside binding, does not get exported from CHO cells. We conclude that the beta-galactoside binding site represents the primary targeting motif of galectins defining a galectin export machinery that makes use of beta-galactoside-containing surface molecules as export receptors for intracellular galectin-1.

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Published date: 24 October 2005
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 200639
URI: http://eprints.soton.ac.uk/id/eprint/200639
ISSN: 1932-6203
PURE UUID: 58f87f7e-cd67-482c-9b5f-5ee90a4f5ea2
ORCID for Ivo Tews: ORCID iD orcid.org/0000-0002-4704-1139

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Date deposited: 01 Nov 2011 15:31
Last modified: 03 Dec 2019 01:41

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