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NMR redox studies of flavocytochrome c(3) from Shewanella frigidimarina

Pessanha, M., Turner, D. L., Rothery, E. L., Pankhurst, K. L., Reid, G. A., Chapman, S. K., Xavier, A. V. and Salgueiro, C. A. (2003) NMR redox studies of flavocytochrome c(3) from Shewanella frigidimarina Inorganica Chimica Acta, 356, pp. 379-381. (doi:10.1016/S0020-1693(03)00179-8).

Record type: Article


Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages.

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Published date: 3 December 2003
Keywords: shewanella, electron tranfer protein, multihaem, flavocytochrome, nmr, paramagnetic shiftsfumarate reductase, cytochrome c(3), desulfovibrio-vulgaris, energytransduction, putrefaciens mr-1, electron-transfer, heme core, cooperativity, respiration, ncimb400


Local EPrints ID: 20064
ISSN: 0020-1693
PURE UUID: 09fe34a1-d9c9-4a2d-ad80-f2b2ffb5cab5

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Date deposited: 23 Feb 2006
Last modified: 17 Jul 2017 16:29

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Author: M. Pessanha
Author: D. L. Turner
Author: E. L. Rothery
Author: K. L. Pankhurst
Author: G. A. Reid
Author: S. K. Chapman
Author: A. V. Xavier
Author: C. A. Salgueiro

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