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Parallel and multiplexed bead-based assays and encoding strategies

Parallel and multiplexed bead-based assays and encoding strategies
Parallel and multiplexed bead-based assays and encoding strategies
Advances in high throughput screening (HTS), together with the rapid progress in combinatorial chemistry, genomic and proteomic sciences have dramatically stimulated the development of a variety tools to enable the drug discovery process to become more efficient. Major future challenges in HTS include obtaining high density and good quality data based on assays that are rapid, reliable, inexpensive, sensitive, simple and miniaturised. This paper reviews the development and role of bead-based assays for HTS including DNA and single nucleotide polymorphism (SNP) assays, particularly from a multiplex perspective and evaluating the recent advances in bead-based arrays. The encoding strategies that are commonly used in bead-based assays are highlighted, while the importance of magnetic beads in genomic and proteomic purifications is discussed. In conclusion, bead-based assays offer a powerful promising approach for many aspects of drug discovery.
scintillation proximity assay, nucleotide polymorphism analysis, human flap endonuclease-1, flow-cytometric analysis, fluorescent microsphereimmunoassay, high-throughput analysis, gene-expression, humancytokines, magnetic beads, simultaneous quantification
1386-2073
577-587
Yingyongnarongkul, B.E.
2e3bfcb5-7fe4-4feb-b0e6-9838db0f06f1
How, S.E.
3b9f8827-5123-4778-b76e-c3196f8b9dbe
Diaz-Mochon, J.J.
9d622400-bf83-4843-967e-f10bf6f3fd5f
Muzerelle, M.
9fde33df-0ef2-4c00-962b-99d42431de85
Bradley, M.
078a34d6-96c4-4ce1-9aa1-454d9ee0030d
Yingyongnarongkul, B.E.
2e3bfcb5-7fe4-4feb-b0e6-9838db0f06f1
How, S.E.
3b9f8827-5123-4778-b76e-c3196f8b9dbe
Diaz-Mochon, J.J.
9d622400-bf83-4843-967e-f10bf6f3fd5f
Muzerelle, M.
9fde33df-0ef2-4c00-962b-99d42431de85
Bradley, M.
078a34d6-96c4-4ce1-9aa1-454d9ee0030d

Yingyongnarongkul, B.E., How, S.E., Diaz-Mochon, J.J., Muzerelle, M. and Bradley, M. (2003) Parallel and multiplexed bead-based assays and encoding strategies. Combinatorial Chemistry & High Throughput Screening, 6 (7), 577-587.

Record type: Article

Abstract

Advances in high throughput screening (HTS), together with the rapid progress in combinatorial chemistry, genomic and proteomic sciences have dramatically stimulated the development of a variety tools to enable the drug discovery process to become more efficient. Major future challenges in HTS include obtaining high density and good quality data based on assays that are rapid, reliable, inexpensive, sensitive, simple and miniaturised. This paper reviews the development and role of bead-based assays for HTS including DNA and single nucleotide polymorphism (SNP) assays, particularly from a multiplex perspective and evaluating the recent advances in bead-based arrays. The encoding strategies that are commonly used in bead-based assays are highlighted, while the importance of magnetic beads in genomic and proteomic purifications is discussed. In conclusion, bead-based assays offer a powerful promising approach for many aspects of drug discovery.

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More information

Published date: 1 November 2003
Keywords: scintillation proximity assay, nucleotide polymorphism analysis, human flap endonuclease-1, flow-cytometric analysis, fluorescent microsphereimmunoassay, high-throughput analysis, gene-expression, humancytokines, magnetic beads, simultaneous quantification

Identifiers

Local EPrints ID: 20109
URI: http://eprints.soton.ac.uk/id/eprint/20109
ISSN: 1386-2073
PURE UUID: ce7180d4-8612-4d90-b493-b080ae562e41

Catalogue record

Date deposited: 23 Feb 2006
Last modified: 07 Jan 2022 22:04

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Contributors

Author: B.E. Yingyongnarongkul
Author: S.E. How
Author: J.J. Diaz-Mochon
Author: M. Muzerelle
Author: M. Bradley

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