The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor
The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor
We report a new approach to the measurement of alkaline phosphatase concentration based on the use of a disposable poly(aniline) microelectrochemical transistor. The measurement is carried out in a two cell configuration in which the poly(aniline) microelectrochemical transistor operates in acid solution and is connected to the alkaline buffer solution containing the analyte by a salt bridge. Disposable microelectrochemical transistors were reproducibly fabricated by electrochemical deposition of poly(aniline) onto photolithographically fabricated gold microband arrays. Using these devices alkaline phosphatase was detected by employing p-aminophenyl phosphate as the substrate for the enzyme and using glucose and glucose oxidase to recycle the p-aminophenol generated upon enzyme catalysed hydrolysis of the phosphate. Recycling the p-aminophenol with glucose and glucose oxidase amplified the detection of alkaline phosphatase approximately tenfold. Using this approach we obtain linear calibration curves for alkaline phosphatase up to 5 nM within 70 s on single use devices.
alkaline phosphatase, nanomolar concentration, disposable, microelectrochemical, transistorchemical derivatization, amperometric detection, composite films, glucose, ph, poly(3-methylthiophene), conductivity, fabrication, polyaniline, hydrogen
53-59
Astier, Y.
71e0ed8d-71bc-42e9-9225-398248c568bf
Bartlett, P.N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
1 August 2004
Astier, Y.
71e0ed8d-71bc-42e9-9225-398248c568bf
Bartlett, P.N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Astier, Y. and Bartlett, P.N.
(2004)
The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor.
Bioelectrochemistry, 64 (1), .
(doi:10.1016/j.bioelechem.2004.01.004).
Abstract
We report a new approach to the measurement of alkaline phosphatase concentration based on the use of a disposable poly(aniline) microelectrochemical transistor. The measurement is carried out in a two cell configuration in which the poly(aniline) microelectrochemical transistor operates in acid solution and is connected to the alkaline buffer solution containing the analyte by a salt bridge. Disposable microelectrochemical transistors were reproducibly fabricated by electrochemical deposition of poly(aniline) onto photolithographically fabricated gold microband arrays. Using these devices alkaline phosphatase was detected by employing p-aminophenyl phosphate as the substrate for the enzyme and using glucose and glucose oxidase to recycle the p-aminophenol generated upon enzyme catalysed hydrolysis of the phosphate. Recycling the p-aminophenol with glucose and glucose oxidase amplified the detection of alkaline phosphatase approximately tenfold. Using this approach we obtain linear calibration curves for alkaline phosphatase up to 5 nM within 70 s on single use devices.
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Published date: 1 August 2004
Keywords:
alkaline phosphatase, nanomolar concentration, disposable, microelectrochemical, transistorchemical derivatization, amperometric detection, composite films, glucose, ph, poly(3-methylthiophene), conductivity, fabrication, polyaniline, hydrogen
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Local EPrints ID: 20122
URI: http://eprints.soton.ac.uk/id/eprint/20122
PURE UUID: abdd7601-4ba5-4eb4-8608-78753f90e3e1
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Date deposited: 22 Feb 2006
Last modified: 16 Mar 2024 02:42
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Author:
Y. Astier
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