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The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor

The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor
The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor
We report a new approach to the measurement of alkaline phosphatase concentration based on the use of a disposable poly(aniline) microelectrochemical transistor. The measurement is carried out in a two cell configuration in which the poly(aniline) microelectrochemical transistor operates in acid solution and is connected to the alkaline buffer solution containing the analyte by a salt bridge. Disposable microelectrochemical transistors were reproducibly fabricated by electrochemical deposition of poly(aniline) onto photolithographically fabricated gold microband arrays. Using these devices alkaline phosphatase was detected by employing p-aminophenyl phosphate as the substrate for the enzyme and using glucose and glucose oxidase to recycle the p-aminophenol generated upon enzyme catalysed hydrolysis of the phosphate. Recycling the p-aminophenol with glucose and glucose oxidase amplified the detection of alkaline phosphatase approximately tenfold. Using this approach we obtain linear calibration curves for alkaline phosphatase up to 5 nM within 70 s on single use devices.
alkaline phosphatase, nanomolar concentration, disposable, microelectrochemical, transistorchemical derivatization, amperometric detection, composite films, glucose, ph, poly(3-methylthiophene), conductivity, fabrication, polyaniline, hydrogen
53-59
Astier, Y.
71e0ed8d-71bc-42e9-9225-398248c568bf
Bartlett, P.N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Astier, Y.
71e0ed8d-71bc-42e9-9225-398248c568bf
Bartlett, P.N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Astier, Y. and Bartlett, P.N. (2004) The measurement of alkaline phosphatase at nanomolar concentration within 70 s using a disposable microelectrochemical transistor. Bioelectrochemistry, 64 (1), 53-59. (doi:10.1016/j.bioelechem.2004.01.004).

Record type: Article

Abstract

We report a new approach to the measurement of alkaline phosphatase concentration based on the use of a disposable poly(aniline) microelectrochemical transistor. The measurement is carried out in a two cell configuration in which the poly(aniline) microelectrochemical transistor operates in acid solution and is connected to the alkaline buffer solution containing the analyte by a salt bridge. Disposable microelectrochemical transistors were reproducibly fabricated by electrochemical deposition of poly(aniline) onto photolithographically fabricated gold microband arrays. Using these devices alkaline phosphatase was detected by employing p-aminophenyl phosphate as the substrate for the enzyme and using glucose and glucose oxidase to recycle the p-aminophenol generated upon enzyme catalysed hydrolysis of the phosphate. Recycling the p-aminophenol with glucose and glucose oxidase amplified the detection of alkaline phosphatase approximately tenfold. Using this approach we obtain linear calibration curves for alkaline phosphatase up to 5 nM within 70 s on single use devices.

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More information

Published date: 1 August 2004
Keywords: alkaline phosphatase, nanomolar concentration, disposable, microelectrochemical, transistorchemical derivatization, amperometric detection, composite films, glucose, ph, poly(3-methylthiophene), conductivity, fabrication, polyaniline, hydrogen

Identifiers

Local EPrints ID: 20122
URI: http://eprints.soton.ac.uk/id/eprint/20122
PURE UUID: abdd7601-4ba5-4eb4-8608-78753f90e3e1
ORCID for P.N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 22 Feb 2006
Last modified: 29 Oct 2019 02:07

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