Spectrofluorimetric determination of surface-bound thiol groups and its application to the analysis of thiol-modified silicas
Spectrofluorimetric determination of surface-bound thiol groups and its application to the analysis of thiol-modified silicas
A sensitive spectofluorimetric method has been developed for the quantitative measurement of surface-bound thiol groups. The procedure is based on the quantitative esterification of the thiol group with Rhodamine B and its subsequent release from the solid by base hydrolysis for spectrofluorimetric determination. Application of the method to the analysis of thiol-modified nanoparticulate silicas yielded results that compared favourably against alternative approaches based on measurements of mercury capacity and iodometric titration of the thiol groups. Non-specific Rhodamine uptake, assessed using unmodified silica and C-18-modified silica, did not significantly influence the analytical results. When applied to a typical 50 mg sample, the detection limit of the procedure was 1 nmole SH g(-1) silica.
in-vitro evaluation, selective preconcentration, sulfhydryl-groups, polymers
860-863
Howard, A.G.
12632e33-1266-49ec-ab18-8ca20c4dee72
Khdary, N.H.
23ed7d6e-62f9-4c0b-b45a-f86ef5e3c766
2004
Howard, A.G.
12632e33-1266-49ec-ab18-8ca20c4dee72
Khdary, N.H.
23ed7d6e-62f9-4c0b-b45a-f86ef5e3c766
Howard, A.G. and Khdary, N.H.
(2004)
Spectrofluorimetric determination of surface-bound thiol groups and its application to the analysis of thiol-modified silicas.
Analyst, 129 (9), .
(doi:10.1039/b407566h).
Abstract
A sensitive spectofluorimetric method has been developed for the quantitative measurement of surface-bound thiol groups. The procedure is based on the quantitative esterification of the thiol group with Rhodamine B and its subsequent release from the solid by base hydrolysis for spectrofluorimetric determination. Application of the method to the analysis of thiol-modified nanoparticulate silicas yielded results that compared favourably against alternative approaches based on measurements of mercury capacity and iodometric titration of the thiol groups. Non-specific Rhodamine uptake, assessed using unmodified silica and C-18-modified silica, did not significantly influence the analytical results. When applied to a typical 50 mg sample, the detection limit of the procedure was 1 nmole SH g(-1) silica.
This record has no associated files available for download.
More information
Published date: 2004
Keywords:
in-vitro evaluation, selective preconcentration, sulfhydryl-groups, polymers
Identifiers
Local EPrints ID: 20237
URI: http://eprints.soton.ac.uk/id/eprint/20237
ISSN: 0003-2654
PURE UUID: b83bdb0b-79ea-46f3-a9e4-bd96c0df7c3b
Catalogue record
Date deposited: 20 Feb 2006
Last modified: 15 Mar 2024 06:23
Export record
Altmetrics
Contributors
Author:
A.G. Howard
Author:
N.H. Khdary
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics