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DNA sequence recognition by an isopropyl substituted thiazole polyamide

James, Peter L., Merkina, Elena E., Khalaf, Abedawn I., Suckling, Colin J., Waigh, Roger D., Brown, Tom and Fox, Keith R. (2004) DNA sequence recognition by an isopropyl substituted thiazole polyamide Nucleic Acids Research, 32, (11), pp. 3410-3417. (doi:10.1093/nar/gkh666).

Record type: Article


We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G.

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Published date: 1 June 2004
Keywords: minor-groove, hairpin polyamide, binding, drug, duplex, specificity, distamycin, netropsin, ligands


Local EPrints ID: 20246
ISSN: 0305-1048
PURE UUID: efc3319c-823f-44a8-8bf6-7c78016a8fa8
ORCID for Keith R. Fox: ORCID iD

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Date deposited: 21 Feb 2006
Last modified: 17 Jul 2017 16:29

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Author: Peter L. James
Author: Elena E. Merkina
Author: Abedawn I. Khalaf
Author: Colin J. Suckling
Author: Roger D. Waigh
Author: Tom Brown
Author: Keith R. Fox ORCID iD

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