Thiamine biosynthesis in Escherichia coli: in vitro reconstitution of the thiazole synthase activity
Thiamine biosynthesis in Escherichia coli: in vitro reconstitution of the thiazole synthase activity
The biosynthesis of thiamine in Escherichia coli requires the formation of an intermediate thiazole from tyrosine, 1-deoxy-D-xylulose-5-phosphate (Dxp), and cysteine using at least six structural proteins, ThiFSGH, IscS, and ThiI. We describe for the first time the reconstitution of thiazole synthase activity using cell-free extracts and proteins derived from adenosine-treated E. coli 83-1 cells. The addition of adenosine or adenine to growing cultures of Aerobacter aerogenes, Salmonella typhimurium, and E. coli has been shown previously to relieve the repression by thiamine of its own biosynthesis and increase the expression levels of the thiamine biosynthetic enzymes. By exploiting this effect, we show that the in vitro thiazole synthase activity of cleared lysates or desalted proteins from E. coli 83-1 cells is dependent upon the addition of purified ThiGH-His complex, tyrosine (but not cysteine or 1-deoxy-D-xylulose-5- phosphate), and an as yet unidentified intermediate present in the protein fraction from these cells. The activity is strongly stimulated by the addition of S-adenosylmethionine and NADPH.
enterica serovar typhimurium, fe-s cluster, salmonella-typhimurium, 5-aminoimidazole ribotide, pyrimidine moiety, nifs gene, protein, identification, precursor, complex
17054-17062
Leonardi, Roberta
d5419843-b210-4d01-a76e-c5a8fceed358
Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
23 April 2004
Leonardi, Roberta
d5419843-b210-4d01-a76e-c5a8fceed358
Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
Leonardi, Roberta and Roach, Peter L.
(2004)
Thiamine biosynthesis in Escherichia coli: in vitro reconstitution of the thiazole synthase activity.
The Journal of Biological Chemistry, 279 (17), .
(doi:10.1074/jbc.M312714200).
Abstract
The biosynthesis of thiamine in Escherichia coli requires the formation of an intermediate thiazole from tyrosine, 1-deoxy-D-xylulose-5-phosphate (Dxp), and cysteine using at least six structural proteins, ThiFSGH, IscS, and ThiI. We describe for the first time the reconstitution of thiazole synthase activity using cell-free extracts and proteins derived from adenosine-treated E. coli 83-1 cells. The addition of adenosine or adenine to growing cultures of Aerobacter aerogenes, Salmonella typhimurium, and E. coli has been shown previously to relieve the repression by thiamine of its own biosynthesis and increase the expression levels of the thiamine biosynthetic enzymes. By exploiting this effect, we show that the in vitro thiazole synthase activity of cleared lysates or desalted proteins from E. coli 83-1 cells is dependent upon the addition of purified ThiGH-His complex, tyrosine (but not cysteine or 1-deoxy-D-xylulose-5- phosphate), and an as yet unidentified intermediate present in the protein fraction from these cells. The activity is strongly stimulated by the addition of S-adenosylmethionine and NADPH.
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Published date: 23 April 2004
Keywords:
enterica serovar typhimurium, fe-s cluster, salmonella-typhimurium, 5-aminoimidazole ribotide, pyrimidine moiety, nifs gene, protein, identification, precursor, complex
Identifiers
Local EPrints ID: 20268
URI: http://eprints.soton.ac.uk/id/eprint/20268
ISSN: 0021-9258
PURE UUID: 89ed654a-32aa-43f0-9cf8-49240dbf9445
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Date deposited: 20 Feb 2006
Last modified: 15 Mar 2024 06:23
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Author:
Roberta Leonardi
Author:
Peter L. Roach
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