The University of Southampton
University of Southampton Institutional Repository

Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA

Record type: Article

We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif, 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) has very high affinity for AT relative to all other Watson-Crick base pairs in DNA. Complexes containing two or more substitutions with this nucleotide analogue are stable at pH 7.0, even though they contain several C.GC base triplets. These modified triplex-forming oligonucleotides retain exquisite sequence specificity, with enhanced discrimination against YR base pairs (especially CG). These properties make BAU a useful base analogue for the sequence-specific creation of stable triple helices at pH 7.0.

Full text not available from this repository.

Citation

Osborne, Sadie D., Powers, Vicki E.C., Rusling, David A., Lack, Oliver, Fox, Keith R. and Brown, Tom (2004) Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA Nucleic Acids Research, 32, (15), pp. 4439-4447. (doi:10.1093/nar/gkh776).

More information

Published date: 2004
Keywords: double-stranded dna, c+center-dot-gc, helix formation, -aminoethoxy-modified oligonucleotides, dual recognition, gene-expression, stability, stabilization, spectroscopy, polyamines

Identifiers

Local EPrints ID: 20299
URI: http://eprints.soton.ac.uk/id/eprint/20299
ISSN: 0305-1048
PURE UUID: ce5948f3-4763-4b03-94a8-3efeded97fee
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 20 Feb 2006
Last modified: 17 Jul 2017 16:29

Export record

Altmetrics

Contributors

Author: Sadie D. Osborne
Author: Vicki E.C. Powers
Author: David A. Rusling
Author: Oliver Lack
Author: Keith R. Fox ORCID iD
Author: Tom Brown

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×