Dendrimers and combinatorial chemistry - tools for fluorescent enhancement in protease assays

Ternon, Michaël L., Diaz-Mochón, Juan José, Belsom, Adam and Bradley, Mark (2004) Dendrimers and combinatorial chemistry - tools for fluorescent enhancement in protease assays. Tetrahedron, 60 (39), 8721-8728. (doi:10.1016/j.tet.2004.05.105).

Record type: Article

Abstract

FRET based systems are some of the best methods available to detect and monitor proteolytic activity. To enhance fluorescent signals and hence assay sensitivity, two different systems were developed using two different dendrimeric constructs. In the first case, a triple branched dendrimer bearing three dansyl groups was used to enhance assay sensitivity and showed a significant enhancement of fluorescence following enzymatic cleavage. In another example, a tris-fluorescein probe, that undergoes self-quenching, was utilized in a combinatorial library synthesis to map the substrate specificity of proteases.

This record has no associated files available for download.

More information

Published date: 20 September 2004

Additional Information: Solid and Solution Phase Combinatorial Chemistry. Symposium-in-Print 107. Edited by Rolf Breinbaur and Herbert Waldmann

Keywords: fret, enzymatic cleavage, substrate specificity, combinatorial libraries, self-quenchingresonance energy-transfer, peptide-synthesis, discovery, identification, libraries, design, probe

Learn more about Chemistry research