The University of Southampton
University of Southampton Institutional Repository

Submicron patterning of DNA oligonucleotides on silicon

Record type: Article

The covalent attachment of DNA oligonucleotides onto crystalline silicon (100) surfaces, in patterns with submicron features, in a straightforward, two-step process is presented. UV light exposure of a hydrogen-terminated silicon (100) surface coated with alkenes functionalized with N-hydroxysuccinimide ester groups resulted in the covalent attachment of the alkene as a monolayer on the surface. Submicron-scale patterning of surfaces was achieved by illumination with an interference pattern obtained by the transmission of 248 nm excimer laser light through a phase mask. The N-hydroxysuccinimide ester surface acted as a template for the subsequent covalent attachment of aminohexyl-modified DNA-oligonucleotides. Oligonucleotide patterns, with feature sizes of 500 nm, were reliably produced over large areas. The patterned surfaces were characterized with atomic force microscopy, scanning electron microscopy, epifluorescence microscopy and ellipsometry. Complementary oligonucleotides were hybridized to the surface-attached oligonucleotides with a density of 7 x 10(12) DNA oligonucleotides per square centimetre. The method will offer much potential for the creation of nano- and micro-scale DNA biosensor devices in silicon.

Full text not available from this repository.

Citation

Yin, H.B., Brown, T., Wilkinson, J.S., Eason, R.W. and Melvin, T. (2004) Submicron patterning of DNA oligonucleotides on silicon Nucleic Acids Research, 32, (14), e118-[7pp]. (doi:10.1093/nar/gnh113).

More information

Published date: 1 August 2004
Keywords: self-assembled monolayers, surfaces, lithography, nanostructures, fabrication, microarrays, 1-alkenes, si(100), si(111), devices

Identifiers

Local EPrints ID: 20346
URI: http://eprints.soton.ac.uk/id/eprint/20346
ISSN: 0305-1048
PURE UUID: 47615dd8-1320-470b-9094-5186c8476c32
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697
ORCID for R.W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 16 Feb 2006
Last modified: 17 Jul 2017 16:29

Export record

Altmetrics

Contributors

Author: H.B. Yin
Author: T. Brown
Author: J.S. Wilkinson ORCID iD
Author: R.W. Eason ORCID iD
Author: T. Melvin

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×