Submicron patterning of DNA oligonucleotides on silicon

Yin, H.B., Brown, T., Wilkinson, J.S., Eason, R.W. and Melvin, T. (2004) Submicron patterning of DNA oligonucleotides on silicon Nucleic Acids Research, 32, (14), e118-[7pp]. (doi:10.1093/nar/gnh113).


Full text not available from this repository.


The covalent attachment of DNA oligonucleotides onto crystalline silicon (100) surfaces, in patterns with submicron features, in a straightforward, two-step process is presented. UV light exposure of a hydrogen-terminated silicon (100) surface coated with alkenes functionalized with N-hydroxysuccinimide ester groups resulted in the covalent attachment of the alkene as a monolayer on the surface. Submicron-scale patterning of surfaces was achieved by illumination with an interference pattern obtained by the transmission of 248 nm excimer laser light through a phase mask. The N-hydroxysuccinimide ester surface acted as a template for the subsequent covalent attachment of aminohexyl-modified DNA-oligonucleotides. Oligonucleotide patterns, with feature sizes of 500 nm, were reliably produced over large areas. The patterned surfaces were characterized with atomic force microscopy, scanning electron microscopy, epifluorescence microscopy and ellipsometry. Complementary oligonucleotides were hybridized to the surface-attached oligonucleotides with a density of 7 x 10(12) DNA oligonucleotides per square centimetre. The method will offer much potential for the creation of nano- and micro-scale DNA biosensor devices in silicon.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1093/nar/gnh113
ISSNs: 0305-1048 (print)
Related URLs:
Keywords: self-assembled monolayers, surfaces, lithography, nanostructures, fabrication, microarrays, 1-alkenes, si(100), si(111), devices
ePrint ID: 20346
Date :
Date Event
1 August 2004Published
Date Deposited: 16 Feb 2006
Last Modified: 16 Apr 2017 22:59
Further Information:Google Scholar

Actions (login required)

View Item View Item