DNA transfection screening from single beads
Yingyongnarongkul, Boon-ek, Howarth, Mark, Elliot, Tim and Bradley, Mark (2004) DNA transfection screening from single beads Journal of Combinatorial Chemistry, 6, (5), pp. 753-760. (doi:10.1021/cc049945t). (PMID:15360210).
- Version of Record
The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one arginine headgroup and a cholesterol tail were found to be the most active, even though their DNA binding strength (retardation assays) was relatively weak. Single-bead screening of transfection activity facilitates the rapid analysis of libraries of transfection reagents and will allow the rapid optimization of gene delivery into cells, both in culture and in vivo.
|Digital Object Identifier (DOI):||doi:10.1021/cc049945t|
|Keywords:||solid-phase construction, high-loading resin, gene delivery, cationic lipids, dendrimer synthesis, parallel synthesis, in-vivo, efficient, complexes, cells|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Date Deposited:||16 Feb 2006|
|Last Modified:||22 Feb 2017 00:09|
Funded by: BBSRC (UNSPECIFIED)
UNSPECIFIED to UNSPECIFIED
|Further Information:||Google Scholar|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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