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DNA transfection screening from single beads

DNA transfection screening from single beads
DNA transfection screening from single beads
The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one arginine headgroup and a cholesterol tail were found to be the most active, even though their DNA binding strength (retardation assays) was relatively weak. Single-bead screening of transfection activity facilitates the rapid analysis of libraries of transfection reagents and will allow the rapid optimization of gene delivery into cells, both in culture and in vivo.
solid-phase construction, high-loading resin, gene delivery, cationic lipids, dendrimer synthesis, parallel synthesis, in-vivo, efficient, complexes, cells
1520-4766
753-760
Yingyongnarongkul, Boon-ek
9fd5bdf6-d2f0-4b32-b1f2-72f1931d9605
Howarth, Mark
9b79b039-8294-4326-be62-4a72b7c60f8e
Elliot, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Yingyongnarongkul, Boon-ek
9fd5bdf6-d2f0-4b32-b1f2-72f1931d9605
Howarth, Mark
9b79b039-8294-4326-be62-4a72b7c60f8e
Elliot, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900

Yingyongnarongkul, Boon-ek, Howarth, Mark, Elliot, Tim and Bradley, Mark (2004) DNA transfection screening from single beads Journal of Combinatorial Chemistry, 6, (5), pp. 753-760. (doi:10.1021/cc049945t). (PMID:15360210).

Record type: Article

Abstract

The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one arginine headgroup and a cholesterol tail were found to be the most active, even though their DNA binding strength (retardation assays) was relatively weak. Single-bead screening of transfection activity facilitates the rapid analysis of libraries of transfection reagents and will allow the rapid optimization of gene delivery into cells, both in culture and in vivo.

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Published date: 2004
Keywords: solid-phase construction, high-loading resin, gene delivery, cationic lipids, dendrimer synthesis, parallel synthesis, in-vivo, efficient, complexes, cells

Identifiers

Local EPrints ID: 20347
URI: http://eprints.soton.ac.uk/id/eprint/20347
ISSN: 1520-4766
PURE UUID: a1ca5f45-7dc3-42f7-b885-85f25f4c6042
ORCID for Tim Elliot: ORCID iD orcid.org/0000-0003-1097-0222

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Date deposited: 16 Feb 2006
Last modified: 17 Jul 2017 16:29

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Contributors

Author: Boon-ek Yingyongnarongkul
Author: Mark Howarth
Author: Tim Elliot ORCID iD
Author: Mark Bradley

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