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DNA transfection screening from single beads

Record type: Article

The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one arginine headgroup and a cholesterol tail were found to be the most active, even though their DNA binding strength (retardation assays) was relatively weak. Single-bead screening of transfection activity facilitates the rapid analysis of libraries of transfection reagents and will allow the rapid optimization of gene delivery into cells, both in culture and in vivo.

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Yingyongnarongkul, Boon-ek, Howarth, Mark, Elliot, Tim and Bradley, Mark (2004) DNA transfection screening from single beads Journal of Combinatorial Chemistry, 6, (5), pp. 753-760. (doi:10.1021/cc049945t). (PMID:15360210).

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Published date: 2004
Keywords: solid-phase construction, high-loading resin, gene delivery, cationic lipids, dendrimer synthesis, parallel synthesis, in-vivo, efficient, complexes, cells


Local EPrints ID: 20347
ISSN: 1520-4766
PURE UUID: a1ca5f45-7dc3-42f7-b885-85f25f4c6042
ORCID for Tim Elliot: ORCID iD

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Date deposited: 16 Feb 2006
Last modified: 17 Jul 2017 16:29

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Author: Boon-ek Yingyongnarongkul
Author: Mark Howarth
Author: Tim Elliot ORCID iD
Author: Mark Bradley

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