DNA transfection screening from single beads

Yingyongnarongkul, Boon-ek, Howarth, Mark, Elliot, Tim and Bradley, Mark (2004) DNA transfection screening from single beads Journal of Combinatorial Chemistry, 6, (5), pp. 753-760. (doi:10.1021/cc049945t). (PMID:15360210).


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The solid-phase synthesis of a library of arginine-containing lipid transfection agents on high-loading beads is described. The transfection activity of the cationic lipids was determined using compound cleaved from single beads (single-bead screening) and showed, in some cases, comparable or higher DNA transfection activities as compared to commercially available reagents. Lipids with one arginine headgroup and a cholesterol tail were found to be the most active, even though their DNA binding strength (retardation assays) was relatively weak. Single-bead screening of transfection activity facilitates the rapid analysis of libraries of transfection reagents and will allow the rapid optimization of gene delivery into cells, both in culture and in vivo.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1021/cc049945t
ISSNs: 1520-4766 (print)
Keywords: solid-phase construction, high-loading resin, gene delivery, cationic lipids, dendrimer synthesis, parallel synthesis, in-vivo, efficient, complexes, cells
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
ePrint ID: 20347
Date :
Date Event
Date Deposited: 16 Feb 2006
Last Modified: 16 Apr 2017 22:59
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/20347

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