The University of Southampton
University of Southampton Institutional Repository

Solid-phase synthesis of 89 polyamine-based cationic lipids for DNA delivery to mammalian cells

Record type: Article

The ability of non-viral gene delivery systems to overcome extracellular and intracellular barriers is a critical issue for future clinical applications of gene therapy. In recent years much effort has been focused on the development of a variety of DNA carriers, and cationic liposomes have become the most common non-viral gene delivery system. Solid-phase synthesis was used to produce three libraries of polyamine-based cationic lipids with diverse hydrophobic tails. These were characterised, and structure-activity relationships were determined for DNA binding and transfection ability of these compounds when formulated as cationic liposomes. Two of the cationic lipids produced high-efficiency transfection of human cells. Surprisingly, these two compounds were from the library with two headgroups and one aliphatic tail, a compound class regarded as detergent-like and little investigated for transfection. These cationic lipids are promising reagents for gene delivery and illustrate the potential of solid-phase synthesis methods for lipoplex discovery.

Full text not available from this repository.


Yingyongnarongkul, Boon-ek, Howarth, Mark, Elliott, Tim and Bradley, Mark (2004) Solid-phase synthesis of 89 polyamine-based cationic lipids for DNA delivery to mammalian cells Chemistry - A European Journal, 10, (2), pp. 463-473. (doi:10.1002/chem.200305232).

More information

Published date: 23 January 2004
Keywords: cationic lipids, combinatorial chemistry, gene therapy, non-viral vector, solid-phase synthesisgene-transfer, liposome complexes, biologic activity, amino-groups, transfection, vectors, efficient, deprotection, guanidines, mechanism


Local EPrints ID: 20348
ISSN: 0947-6539
PURE UUID: 18d14388-eedd-46b9-821d-f20561a96298
ORCID for Tim Elliott: ORCID iD

Catalogue record

Date deposited: 16 Feb 2006
Last modified: 17 Jul 2017 16:29

Export record



Author: Boon-ek Yingyongnarongkul
Author: Mark Howarth
Author: Tim Elliott ORCID iD
Author: Mark Bradley

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.