Analysis of the immune response to Neisseria meningitidis using a proteomics approach
Analysis of the immune response to Neisseria meningitidis using a proteomics approach
The availability of Neisseria genome sequences together with improvements in proteomic technologies provide the opportunity to study at high resolution the immune response to Neisseria meningitidis. In this chapter, we describe a protocol that combines two-dimensional (2D) SDS-PAGE of meningococcal outer membranes with western blotting of human antisera to identify proteins associated with the development of protective antibody responses. This methodology can identify putative vaccine candidates for incorporation in a multi-component serogroup B meningococcal vaccine.
343-360
Williams, Jeanette N.
b8cee39f-8709-40ac-84ad-e62e3c1c7d8e
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31
19 October 2011
Williams, Jeanette N.
b8cee39f-8709-40ac-84ad-e62e3c1c7d8e
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Heckels, John E.
fcfcfafe-5ca8-4728-9c5e-cb67f9af7e31
Williams, Jeanette N., Christodoulides, Myron and Heckels, John E.
(2011)
Analysis of the immune response to Neisseria meningitidis using a proteomics approach.
Methods in Molecular Biology, 799, .
(doi:10.1007/978-1-61779-346-2_20).
(PMID:21993655)
Abstract
The availability of Neisseria genome sequences together with improvements in proteomic technologies provide the opportunity to study at high resolution the immune response to Neisseria meningitidis. In this chapter, we describe a protocol that combines two-dimensional (2D) SDS-PAGE of meningococcal outer membranes with western blotting of human antisera to identify proteins associated with the development of protective antibody responses. This methodology can identify putative vaccine candidates for incorporation in a multi-component serogroup B meningococcal vaccine.
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Published date: 19 October 2011
Organisations:
Clinical & Experimental Sciences
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Local EPrints ID: 203575
URI: http://eprints.soton.ac.uk/id/eprint/203575
PURE UUID: f35b7999-9fc6-4d12-814c-aec71067dfca
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Date deposited: 17 Nov 2011 15:46
Last modified: 15 Mar 2024 02:39
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Author:
Jeanette N. Williams
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