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Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK

Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK
Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK
Prorocentrum lima was isolated from the coastal Fleet lagoon, Dorset, UK in 2000 and a number of clonal cultures established. These were analyzed for okadaic acid (OA), dinophysistoxin-1 (DTX-1), DTX-2, DTX-4 and diol esters by liquid chromatography coupled to mass spectrometry. OA concentrations varied from 0.4 to 17.1 pg OA cell?1 and DTX-1 from 0.4 to 11.3 pg DTX-1 cell?1; DTX-2 was not detected in these isolates. OA and DTX-1 were detected in the culture media, as a result of toxin excretion. DTX-4 and a selection of DTX-4 diol esters were identified using selected ion monitoring, although not all strains produced these compounds. Cell size and number of marginal and valve pores of each strain were observed using scanning electron microscopy. OA and DTX-1 concentrations, pigment content and changes in nitrate and phosphate concentrations in the culture media were followed during growth of one strain of P. lima in batch culture. Diarrhetic shellfish poisoning (DSP) toxins have been previously detected in shellfish cultivated in the Fleet lagoon, but in the absence of any Dinophysis sp. cells. The identification of toxic P. lima strains from the Fleet suggests that this dinoflagellate is the most probable source of occasional DSP detected in the lagoon.
Prorocentrum lima, okadaic acid, Dinophysistoxin-1, diarrhetic shellfish poisoning, pigments
0041-0101
633-649
Nascimento, S.M.
f737e98c-5435-45d0-acd2-dd3440b612d0
Purdie, D.A.
18820b32-185a-467a-8019-01f245191cd8
Morris, S.
7e35be34-5a1e-47de-974d-663982a295cf
Nascimento, S.M.
f737e98c-5435-45d0-acd2-dd3440b612d0
Purdie, D.A.
18820b32-185a-467a-8019-01f245191cd8
Morris, S.
7e35be34-5a1e-47de-974d-663982a295cf

Nascimento, S.M., Purdie, D.A. and Morris, S. (2005) Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK. Toxicon, 45 (5), 633-649. (doi:10.1016/j.toxicon.2004.12.023).

Record type: Article

Abstract

Prorocentrum lima was isolated from the coastal Fleet lagoon, Dorset, UK in 2000 and a number of clonal cultures established. These were analyzed for okadaic acid (OA), dinophysistoxin-1 (DTX-1), DTX-2, DTX-4 and diol esters by liquid chromatography coupled to mass spectrometry. OA concentrations varied from 0.4 to 17.1 pg OA cell?1 and DTX-1 from 0.4 to 11.3 pg DTX-1 cell?1; DTX-2 was not detected in these isolates. OA and DTX-1 were detected in the culture media, as a result of toxin excretion. DTX-4 and a selection of DTX-4 diol esters were identified using selected ion monitoring, although not all strains produced these compounds. Cell size and number of marginal and valve pores of each strain were observed using scanning electron microscopy. OA and DTX-1 concentrations, pigment content and changes in nitrate and phosphate concentrations in the culture media were followed during growth of one strain of P. lima in batch culture. Diarrhetic shellfish poisoning (DSP) toxins have been previously detected in shellfish cultivated in the Fleet lagoon, but in the absence of any Dinophysis sp. cells. The identification of toxic P. lima strains from the Fleet suggests that this dinoflagellate is the most probable source of occasional DSP detected in the lagoon.

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More information

Published date: 2005
Keywords: Prorocentrum lima, okadaic acid, Dinophysistoxin-1, diarrhetic shellfish poisoning, pigments

Identifiers

Local EPrints ID: 20392
URI: https://eprints.soton.ac.uk/id/eprint/20392
ISSN: 0041-0101
PURE UUID: 6cd2cda5-49ea-49e0-95ee-2d4e9de9389a

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Date deposited: 23 Feb 2006
Last modified: 17 Jul 2017 16:28

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