The University of Southampton
University of Southampton Institutional Repository

Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK

Record type: Article

Prorocentrum lima was isolated from the coastal Fleet lagoon, Dorset, UK in 2000 and a number of clonal cultures established. These were analyzed for okadaic acid (OA), dinophysistoxin-1 (DTX-1), DTX-2, DTX-4 and diol esters by liquid chromatography coupled to mass spectrometry. OA concentrations varied from 0.4 to 17.1 pg OA cell?1 and DTX-1 from 0.4 to 11.3 pg DTX-1 cell?1; DTX-2 was not detected in these isolates. OA and DTX-1 were detected in the culture media, as a result of toxin excretion. DTX-4 and a selection of DTX-4 diol esters were identified using selected ion monitoring, although not all strains produced these compounds. Cell size and number of marginal and valve pores of each strain were observed using scanning electron microscopy. OA and DTX-1 concentrations, pigment content and changes in nitrate and phosphate concentrations in the culture media were followed during growth of one strain of P. lima in batch culture. Diarrhetic shellfish poisoning (DSP) toxins have been previously detected in shellfish cultivated in the Fleet lagoon, but in the absence of any Dinophysis sp. cells. The identification of toxic P. lima strains from the Fleet suggests that this dinoflagellate is the most probable source of occasional DSP detected in the lagoon.

Full text not available from this repository.

Citation

Nascimento, S.M., Purdie, D.A. and Morris, S. (2005) Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK Toxicon, 45, (5), pp. 633-649. (doi:10.1016/j.toxicon.2004.12.023).

More information

Published date: 2005
Keywords: Prorocentrum lima, okadaic acid, Dinophysistoxin-1, diarrhetic shellfish poisoning, pigments

Identifiers

Local EPrints ID: 20392
URI: http://eprints.soton.ac.uk/id/eprint/20392
ISSN: 0041-0101
PURE UUID: 6cd2cda5-49ea-49e0-95ee-2d4e9de9389a

Catalogue record

Date deposited: 23 Feb 2006
Last modified: 17 Jul 2017 16:28

Export record

Altmetrics

Contributors

Author: S.M. Nascimento
Author: D.A. Purdie
Author: S. Morris

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×