The University of Southampton
University of Southampton Institutional Repository

Interleukin-13, but not indomethacin, increases cysteinyl-leukotriene synthesis in human lung macrophages

Jackson, Sarah E., Holloway, John W., Warner, Jane A. and Sampson, Anthony P. (2011) Interleukin-13, but not indomethacin, increases cysteinyl-leukotriene synthesis in human lung macrophages Journal of Allergy, 2012, pp. 1-6. (doi:10.1155/2012/348741). (PMID:22121385).

Record type: Article

Abstract

Aspirin-exacerbated respiratory disease (AERD) is associated with constitutively elevated synthesis of bronchoconstrictor cysteinyl-leukotrienes, associated with increased expression of leukotriene (LT)C(4) synthase and Th2 cytokines and airway eosinophilia. We examined whether interleukin-13 can increase LTC(4) synthase gene transcription and cysteinyl-leukotriene synthesis in macrophages isolated from resected human lung tissue and whether an NSAID (indomethacin) can trigger further cysteinyl-leukotriene synthesis in these cells. Overnight culture of human lung macrophages with IL-13 (10?ng/mL) increased spontaneous and ionophore-stimulated production of cysteinyl-leukotrienes by 42% (P = 0.02) and 52% (P = 0.005), respectively, as quantified by enzyme immunoassays, but PCR gene transcription assays did not demonstrate an effect on LTC4S mRNA. The addition of indomethacin (100??M) did not modulate cysteinyl-leukotriene production in either IL-13-treated or untreated macrophages. We conclude that while IL-13 enhances cysteinyl-leukotriene synthesis in human lung macrophages, it does not replicate the enhanced LTC(4) synthase expression observed in the AERD lung nor confer sensitivity to NSAIDs

Full text not available from this repository.

More information

e-pub ahead of print date: 29 October 2011
Organisations: Human Development & Health, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 204877
URI: http://eprints.soton.ac.uk/id/eprint/204877
ISSN: 0021-8707
PURE UUID: ef9e651e-37d6-4d37-a2db-7a7ada3950e1
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 30 Nov 2011 14:43
Last modified: 18 Jul 2017 11:05

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×