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Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear

Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear
Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear
Targeted delivery of treatment agents to the inner ear using nanoparticles is an advanced therapeutic approach to cure or alleviate hearing loss. Designed to target the outer hair cells of the cochlea, two 12-mer peptides (A665 and A666) with affinity to prestin were identified following 3 rounds of sequential phage display. Two-round display with immobilized prestin protein was used to enrich the library for full-length prestin. The last round was performed using Cos-7 cells transiently transfected with a cCFP-prestin plasmid to display phages expressing peptides restrictive to the extracellular loops of prestin. The binding properties of A665 and A666 shown by flow cytometry demonstrated selectivity to prestin-expressing Chinese hamster ovary cells. PEG6K-b-PCL19K polymersomes covalently labelled with these peptides demonstrated effective targeting to outer hair cells in a rat cochlear explant study.
0378-5173
Surovtesva, Elena V.
290f9c6b-aa58-4aba-b767-4f5c75e3c0b1
Johnston, Alexander H.
b3325b84-67c4-4d9c-ba97-57f2fdf017ba
Zhang, Weikai
d742f246-a5f8-4b4f-b249-7676d4769fa7
Zhang, Ya
1820fb16-8a49-4749-9964-12bcbded2641
Kim, Adele
9ecc2e5d-67bb-49f6-8dd7-20ab24f6b9ce
Murakoshi, Michio
263707cb-db67-49a4-b8de-d650660c97a1
Wada, Hiroshi
fde434ee-dc49-4369-b243-90f21756fe48
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Zou, Jing
00c594ea-0fc7-447c-88af-014ee31f1454
Pyykko, Illmari
92cf23a4-83a1-4483-9635-4b420d1ca40a
Surovtesva, Elena V.
290f9c6b-aa58-4aba-b767-4f5c75e3c0b1
Johnston, Alexander H.
b3325b84-67c4-4d9c-ba97-57f2fdf017ba
Zhang, Weikai
d742f246-a5f8-4b4f-b249-7676d4769fa7
Zhang, Ya
1820fb16-8a49-4749-9964-12bcbded2641
Kim, Adele
9ecc2e5d-67bb-49f6-8dd7-20ab24f6b9ce
Murakoshi, Michio
263707cb-db67-49a4-b8de-d650660c97a1
Wada, Hiroshi
fde434ee-dc49-4369-b243-90f21756fe48
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Zou, Jing
00c594ea-0fc7-447c-88af-014ee31f1454
Pyykko, Illmari
92cf23a4-83a1-4483-9635-4b420d1ca40a

Surovtesva, Elena V., Johnston, Alexander H., Zhang, Weikai, Zhang, Ya, Kim, Adele, Murakoshi, Michio, Wada, Hiroshi, Newman, Tracey A., Zou, Jing and Pyykko, Illmari (2012) Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear. International Journal of Pharmaceutics. (doi:10.1016/j.ijpharm.2011.12.042). (PMID:22227343)

Record type: Article

Abstract

Targeted delivery of treatment agents to the inner ear using nanoparticles is an advanced therapeutic approach to cure or alleviate hearing loss. Designed to target the outer hair cells of the cochlea, two 12-mer peptides (A665 and A666) with affinity to prestin were identified following 3 rounds of sequential phage display. Two-round display with immobilized prestin protein was used to enrich the library for full-length prestin. The last round was performed using Cos-7 cells transiently transfected with a cCFP-prestin plasmid to display phages expressing peptides restrictive to the extracellular loops of prestin. The binding properties of A665 and A666 shown by flow cytometry demonstrated selectivity to prestin-expressing Chinese hamster ovary cells. PEG6K-b-PCL19K polymersomes covalently labelled with these peptides demonstrated effective targeting to outer hair cells in a rat cochlear explant study.

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More information

e-pub ahead of print date: 30 December 2011
Published date: March 2012
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 206635
URI: http://eprints.soton.ac.uk/id/eprint/206635
ISSN: 0378-5173
PURE UUID: 4b549fcc-55dc-4757-9c35-c135cb9ec64f
ORCID for Tracey A. Newman: ORCID iD orcid.org/0000-0002-3727-9258

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Date deposited: 09 Jan 2012 16:52
Last modified: 18 Feb 2021 16:46

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Contributors

Author: Elena V. Surovtesva
Author: Alexander H. Johnston
Author: Weikai Zhang
Author: Ya Zhang
Author: Adele Kim
Author: Michio Murakoshi
Author: Hiroshi Wada
Author: Jing Zou
Author: Illmari Pyykko

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