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Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear

Surovtesva, Elena V., Johnston, Alexander H., Zhang, Weikai, Zhang, Ya, Kim, Adele, Murakoshi, Michio, Wada, Hiroshi, Newman, Tracey A., Zou, Jing and Pyykko, Illmari (2012) Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear International Journal of Pharmaceutics (doi:10.1016/j.ijpharm.2011.12.042). (PMID:22227343).

Record type: Article

Abstract

Targeted delivery of treatment agents to the inner ear using nanoparticles is an advanced therapeutic approach to cure or alleviate hearing loss. Designed to target the outer hair cells of the cochlea, two 12-mer peptides (A665 and A666) with affinity to prestin were identified following 3 rounds of sequential phage display. Two-round display with immobilized prestin protein was used to enrich the library for full-length prestin. The last round was performed using Cos-7 cells transiently transfected with a cCFP-prestin plasmid to display phages expressing peptides restrictive to the extracellular loops of prestin. The binding properties of A665 and A666 shown by flow cytometry demonstrated selectivity to prestin-expressing Chinese hamster ovary cells. PEG6K-b-PCL19K polymersomes covalently labelled with these peptides demonstrated effective targeting to outer hair cells in a rat cochlear explant study.

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More information

e-pub ahead of print date: 30 December 2011
Published date: March 2012
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 206635
URI: http://eprints.soton.ac.uk/id/eprint/206635
ISSN: 0378-5173
PURE UUID: 4b549fcc-55dc-4757-9c35-c135cb9ec64f

Catalogue record

Date deposited: 09 Jan 2012 16:52
Last modified: 18 Jul 2017 10:51

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Contributors

Author: Elena V. Surovtesva
Author: Alexander H. Johnston
Author: Weikai Zhang
Author: Ya Zhang
Author: Adele Kim
Author: Michio Murakoshi
Author: Hiroshi Wada
Author: Jing Zou
Author: Illmari Pyykko

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