Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear


Surovtesva, Elena V., Johnston, Alexander H., Zhang, Weikai, Zhang, Ya, Kim, Adele, Murakoshi, Michio, Wada, Hiroshi, Newman, Tracey A., Zou, Jing and Pyykko, Illmari (2011) Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear International Journal of Pharmaceutics (doi:10.1016/j.ijpharm.2011.12.042). (PMID:22227343).

Download

Full text not available from this repository.

Description/Abstract

Targeted delivery of treatment agents to the inner ear using nanoparticles is an advanced therapeutic approach to cure or alleviate hearing loss. Designed to target the outer hair cells of the cochlea, two 12-mer peptides (A665 and A666) with affinity to prestin were identified following 3 rounds of sequential phage display. Two-round display with immobilized prestin protein was used to enrich the library for full-length prestin. The last round was performed using Cos-7 cells transiently transfected with a cCFP-prestin plasmid to display phages expressing peptides restrictive to the extracellular loops of prestin. The binding properties of A665 and A666 shown by flow cytometry demonstrated selectivity to prestin-expressing Chinese hamster ovary cells. PEG6K-b-PCL19K polymersomes covalently labelled with these peptides demonstrated effective targeting to outer hair cells in a rat cochlear explant study.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.ijpharm.2011.12.042
ISSNs: 0378-5173 (print)
Subjects:
Organisations: Faculty of Medicine
ePrint ID: 206635
Date :
Date Event
30 December 2011e-pub ahead of print
March 2012Published
Date Deposited: 09 Jan 2012 16:52
Last Modified: 18 Apr 2017 00:40
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/206635

Actions (login required)

View Item View Item